Send to

Choose Destination
Cent Eur J Immunol. 2017;42(4):331-335. doi: 10.5114/ceji.2017.72826. Epub 2017 Dec 30.

The decrease in number of splenic lymphocytes in mice fed Rhodiola kirilowii during pregnancy and lactation concerns mainly CD19+ and CD4+ cells.

Author information

Department of Regenerative Medicine and Cell Biology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
Pathomorphology Department, Center for Biostructure Research, Warsaw Medical University, Warsaw, Poland.


In previous work we described the decline in the number of splenocytes of mice which during pregnancy and lactation were fed Rhodiola kirilowii. In this work we present the size of individual subpopulations of splenic lymphocytes in these mice. Experiments were performed on adult inbred female Balb/c mice, 8-9 weeks old, 20-22 g b.m., mated with adult males from the same strain. Females, from when the copulatory plug was noted up to the 28th day after delivery, were supplemented daily with lyophilized aqueous (RKW) or 50% hydro-ethanolic (RKW-A) extract (20 mg/kg b.m.) dissolved in distilled water. Then, mice were euthanized, spleens dissected, cells counted and the total numbers of CD3+, CD19+, CD4+, CD8+ and CD335+ splenic lymphocytes were evaluated by cytometry. The number of CD3+ lymphocytes per 1 g of splenic tissue was higher in RKW-A than in RKW spleens and did not differ from the control. The number of CD3+ lymphocytes in RKW spleens was lower than in the controls. The number of CD19+ and CD4+ cells was lower in both experimental groups than in the controls. The number of CD335+(NK) cells was lower in RKW spleens than in the control.


Rhodiola kirilowii; lymphocyte populations; mice; pregnancy; spleen

Conflict of interest statement

The authors declare no conflict of interest.

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center