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Lasers Med Sci. 2018 Apr;33(3):637-645. doi: 10.1007/s10103-018-2455-3. Epub 2018 Feb 21.

Activation of Wnt/β-catenin signaling is involved in hair growth-promoting effect of 655-nm red light and LED in in vitro culture model.

Author information

1
Department of Dermatology, The Third Affiliated Hospital, Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou, 510630, China.
2
State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, No. 381 Wushan Road, Guangzhou, 510640, China.
3
Department of Hair Transplantation, Yuexiu Plastic Surgery Hospital, No.133 Guangzhou Road, Guangzhou, 510601, China.
4
Department of Dermatology, The Third Affiliated Hospital, Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou, 510630, China. wanmj@mail.sysu.edu.cn.

Abstract

Activation of the Wnt/β-catenin signaling pathway plays an important role in hair follicle morphogenesis and hair growth. Recently, low-level laser therapy (LLLT) was evaluated for stimulating hair growth in numerous clinical studies, in which 655-nm red light was found to be most effective and practical for stimulating hair growth. We evaluated whether 655-nm red light + light-emitting diode (LED) could promote human hair growth by activating Wnt/β-catenin signaling. An in vitro culture of human hair follicles (HFs) was irradiated with different intensities of 655-nm red light + LED, 21 h7 (an inhibitor of β-catenin), or both. Immunofluorescence staining was performed to assess the expression of β-catenin, GSK3β, p-GSK3β, and Lef1 in the Wnt/β-catenin signaling. The 655-nm red light + LED not only enhanced hair shaft elongation, but also reduced catagen transition in human hair follicle organ culture, with the greatest effectiveness observed at 5 min (0.839 J/cm2). Additionally, 655-nm red light + LED enhanced the expression of β-catenin, p-GSK3β, and Lef1, signaling molecules of the Wnt/β-catenin pathway, in the hair matrix. Activation of Wnt/β-catenin signaling is involved in hair growth-promoting effect of 655-nm red light and LED in vitro and therefore may serve as an alternative therapeutic option for alopecia.

KEYWORDS:

655 nm; Hair; Low-level laser therapy; Wnt/β-catenin signaling

PMID:
29468283
DOI:
10.1007/s10103-018-2455-3
[Indexed for MEDLINE]

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