Format

Send to

Choose Destination
Cell Rep. 2018 Feb 20;22(8):2026-2038. doi: 10.1016/j.celrep.2018.01.084.

Spine Patterning Is Guided by Segmentation of the Notochord Sheath.

Author information

1
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
2
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA; Department of Dermatology, Duke University Medical Center, Durham, NC 27710, USA.
3
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA; Regeneration Next, Duke University, Durham, NC 27710, USA.
4
Hubrecht Institute - KNAW & UMC Utrecht, 3584 CT, Utrecht, the Netherlands.
5
Hubrecht Institute - KNAW & UMC Utrecht, 3584 CT, Utrecht, the Netherlands; Institute for Cardiovascular Organogenesis and Regeneration, Faculty of Medicine, WWU Münster, 48149 Münster, Germany; CiM Cluster of Excellence (EXC1003-CiM), 48149 Münster, Germany.
6
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA; Regeneration Next, Duke University, Durham, NC 27710, USA. Electronic address: m.bagnat@cellbio.duke.edu.

Abstract

The spine is a segmented axial structure made of alternating vertebral bodies (centra) and intervertebral discs (IVDs) assembled around the notochord. Here, we show that, prior to centra formation, the outer epithelial cell layer of the zebrafish notochord, the sheath, segments into alternating domains corresponding to the prospective centra and IVD areas. This process occurs sequentially in an anteroposterior direction via the activation of Notch signaling in alternating segments of the sheath, which transition from cartilaginous to mineralizing domains. Subsequently, osteoblasts are recruited to the mineralized domains of the notochord sheath to form mature centra. Tissue-specific manipulation of Notch signaling in sheath cells produces notochord segmentation defects that are mirrored in the spine. Together, our findings demonstrate that notochord sheath segmentation provides a template for vertebral patterning in the zebrafish spine.

KEYWORDS:

Notch; and zebrafish; intervertebral disc; notochord sheath; segmentation; spine; vertebrae

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center