Format

Send to

Choose Destination
Phytother Res. 2018 Jun;32(6):1080-1089. doi: 10.1002/ptr.6047. Epub 2018 Feb 21.

Time-dependent effect of phytocannabinoid treatments in fat cells.

Author information

1
Department of Biochemistry and Microbiology, Nelson Mandela Metropolitan University, PO Box 77000, 6031, Port Elizabeth, South Africa.

Abstract

The objectives of this paper is to investigate, demonstrate, and compare the mechanism of action of phytocannabinoids as antidiabetic and anti-obesity agents in preadipocytes and adipocytes, relative to rosiglitazone and metformin. Briefly, cannabis extract, Δ9 -tetrahydrocannabinol and cannabidiol (in very low dosages) were shown to promote glucose uptake higher or to equivalent levels, reduce fat accumulation, and reverse the insulin-resistant state of 3T3-L1 cells more effectively, relative to rosiglitazone and metformin. The phytocannabinoids had a more pronounced effect in preadipocytes undifferentiated model rather than the differentiated model. They induced a protective effect at the mitochondrial level by preventing overactivity of the succinate dehydrogenase pathway (p < .01), unlike rosiglitazone, through activation of the glycerol-3-phosphate dehydrogenase shuttling system. An increase in oxygen consumption and an increased expression of beta to alpha adrenoceptors (p < .05) in treated cells were noted. These findings contribute toward understanding the mechanism of action of phytocannabinoids in fat cells and highlight the antidiabetic and anti-obesity properties of various phytocannabinoids that could potentially support the treatment of obesity-related insulin resistance.

KEYWORDS:

beta/alpha adrenoceptors; glycerol-3-phosphate dehydrogenase shuttling system; insulin-resistance; obesity; phytocannabinoids

PMID:
29464872
DOI:
10.1002/ptr.6047
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center