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Curr Genet. 2018 Aug;64(4):799-806. doi: 10.1007/s00294-018-0812-1. Epub 2018 Feb 20.

The interplay of histone H2B ubiquitination with budding and fission yeast heterochromatin.

Author information

1
Department of Biochemistry and Molecular Genetics, University of Colorado, Denver - School of Medicine, 12801 E. 17th Ave., Aurora, CO, 80045, USA.
2
Department of Biochemistry and Molecular Genetics, University of Colorado, Denver - School of Medicine, 12801 E. 17th Ave., Aurora, CO, 80045, USA. aaron.m.johnson@ucdenver.edu.

Abstract

Mono-ubiquitinated histone H2B (H2B-Ub) is important for chromatin regulation of transcription, chromatin assembly, and also influences heterochromatin. In this review, we discuss the effects of H2B-Ub from nucleosome to higher-order chromatin structure. We then assess what is currently known of the role of H2B-Ub in heterochromatic silencing in budding and fission yeasts (S. cerevisiae and S. pombe), which have distinct silencing mechanisms. In budding yeast, the SIR complex initiates heterochromatin assembly with the aid of a H2B-Ub deubiquitinase, Ubp10. In fission yeast, the RNAi-dependent pathway initiates heterochromatin in the context of low H2B-Ub. We examine how the different silencing machineries overcome the challenge of H2B-Ub chromatin and highlight the importance of using these microorganisms to further our understanding of H2B-Ub in heterochromatic silencing pathways.

KEYWORDS:

Epigenetics; Heterochromatin; SIR complex; Silencing; Ubiquitination; Ubp10

PMID:
29464330
PMCID:
PMC6039258
[Available on 2019-08-01]
DOI:
10.1007/s00294-018-0812-1
[Indexed for MEDLINE]

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