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Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2490-2495. doi: 10.1073/pnas.1715713115. Epub 2018 Feb 20.

The secreted metabolome of Streptomyces chartreusis and implications for bacterial chemistry.

Author information

1
Applied Microbiology, Ruhr University Bochum, 44780 Bochum, Germany.
2
Center for Biotechnology, Bielefeld University, 33594 Bielefeld, Germany.
3
Department of Chemistry, University of Prince Edward Island, PE C1A 4P3 Charlottetown, Canada.
4
Department of General and Molecular Botany, Ruhr University Bochum, 44780 Bochum, Germany.
5
Applied Microbiology, Ruhr University Bochum, 44780 Bochum, Germany; julia.bandow@rub.de.

Abstract

Actinomycetes are known for producing diverse secondary metabolites. Combining genomics with untargeted data-dependent tandem MS and molecular networking, we characterized the secreted metabolome of the tunicamycin producer Streptomyces chartreusis NRRL 3882. The genome harbors 128 predicted biosynthetic gene clusters. We detected >1,000 distinct secreted metabolites in culture supernatants, only 22 of which were identified based on standards and public spectral libraries. S. chartreusis adapts the secreted metabolome to cultivation conditions. A number of metabolites are produced iron dependently, among them 17 desferrioxamine siderophores aiding in iron acquisition. Eight previously unknown members of this long-known compound class are described. A single desferrioxamine synthesis gene cluster was detected in the genome, yet different sets of desferrioxamines are produced in different media. Additionally, a polyether ionophore, differentially produced by the calcimycin biosynthesis cluster, was discovered. This illustrates that metabolite output of a single biosynthetic machine can be exquisitely regulated not only with regard to product quantity but also with regard to product range. Compared with chemically defined medium, in complex medium, total metabolite abundance was higher, structural diversity greater, and the average molecular weight almost doubled. Tunicamycins, for example, were only produced in complex medium. Extrapolating from this study, we anticipate that the larger part of bacterial chemistry, including chemical structures, ecological functions, and pharmacological potential, is yet to be uncovered.

KEYWORDS:

antibiotics; metabolomics; secondary metabolites; siderophores

PMID:
29463727
PMCID:
PMC5877972
DOI:
10.1073/pnas.1715713115
[Indexed for MEDLINE]
Free PMC Article

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