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Fish Shellfish Immunol. 2018 Apr;75:357-364. doi: 10.1016/j.fsi.2018.02.025. Epub 2018 Feb 17.

Dietary administration effects of fenugreek seeds on skin mucosal antioxidant and immunity status of gilthead seabream (Sparus aurata L.).

Author information

1
Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), University of Porto, Terminal de Cruzeiros Do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208, Porto, Portugal; Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain. Electronic address: fguardiola@ciimar.up.pt.
2
Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
3
Department of Food Science, Faculty of Veterinary, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Murcia, Spain; CIBEROBN (CB12/03/30038 Physiopathology of Obesity and Nutrition), Instituto de Salud Carlos III (ISCIII), Madrid, Spain.
4
Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain. Electronic address: aesteban@um.es.

Abstract

Improving fish defense through the preventive administration of immunostimulants has an important role in controlling the outbreak of the disease in aquaculture. As a continuity of our previous studies, this paper describes the effects of dietary fenugreek (Trigonella foenum graecum) seeds on the skin mucosal antioxidant status and immune response of gilthead seabream (Sparus aurata L.). Fish were fed with four experimental diets: one a basal diet (control) and three diets with powdered fenugreek seeds incorporated in the fish feed at 1%, 5%, and 10%. After eight weeks of feeding, free radicals scavenging and antioxidant assays were assessed in skin mucus by measuring the peroxidation of phospholipid liposomes, hydroxyl radical and hydrogen peroxide scavenging, measurement of total antioxidant activity and the determination of antioxidant activity in a linoleic acid system. The skin mucosal immune response was evaluated by measuring the IgM levels and some enzymatic activities (peroxidase, antiprotease, protease, esterase, and ceruloplasmin). Our results demonstrated that fenugreek inclusion improved the hydroxyl radical scavenging capacity and conferred very high antioxidant activity. Besides, only the highest supplementation level (10%) was able to augment the peroxidase and protease activities confronted by a general decrement in the antiprotease activity in the experimental fed groups with 1% and 10%. These results suggest that the dietary administration of fenugreek at the higher inclusion dose enhances the skin mucosal immunity response and the antioxidant status of gilthead seabream a species with one of the highest rates of production in marine aquaculture.

KEYWORDS:

Antioxidant status; Fenugreek seeds; Gilthead seabream (Sparus aurata; Herbal immunostimulants; L); Mucosal immunity; Skin; Teleosts

PMID:
29462748
DOI:
10.1016/j.fsi.2018.02.025
[Indexed for MEDLINE]

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