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Biotechnol Bioeng. 2018 Jun;115(6):1427-1436. doi: 10.1002/bit.26573. Epub 2018 Mar 8.

Fluorescent nanodiamond-bacteriophage conjugates maintain host specificity.

Author information

1
Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas.
2
Center for Phage Technology, Texas A&M University, College Station, Texas.
3
Institute for Quantum Science and Engineering, Texas A&M University, College Station, Texas.
4
Department of Physics and Astronomy, Texas A&M University, College Station, Texas.
5
The National Center for Applied Physics, Riyadh, Saudi Arabia.
6
Columbus NanoWorks, Inc., Columbus, Ohio.
7
Department of Physics, Baylor University, Waco, Texas.
8
Department of Electrical and Computer Engineering, Texas A&M University, College Station, Texas.

Abstract

Rapid identification of specific bacterial strains within clinical, environmental, and food samples can facilitate the prevention and treatment of disease. Fluorescent nanodiamonds (FNDs) are being developed as biomarkers in biology and medicine, due to their excellent imaging properties, ability to accept surface modifications, and lack of toxicity. Bacteriophages, the viruses of bacteria, can have exquisite specificity for certain hosts. We propose to exploit the properties of FNDs and phages to develop phages conjugated with FNDs as long-lived fluorescent diagnostic reagents. In this study, we develop a simple procedure to create such fluorescent probes by functionalizing the FNDs and phages with streptavidin and biotin, respectively. We find that the FND-phage conjugates retain the favorable characteristics of the individual components and can discern their proper host within a mixture. This technology may be further explored using different phage/bacteria systems, different FND color centers and alternate chemical labeling schemes for additional means of bacterial identification and new single-cell/virus studies.

KEYWORDS:

bacteria identification; bacteriophage; conjugation; fluorescent nanodiamonds; live-cell imaging

PMID:
29460442
PMCID:
PMC5912989
[Available on 2019-06-01]
DOI:
10.1002/bit.26573

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