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Food Chem Toxicol. 2018 Apr;114:128-140. doi: 10.1016/j.fct.2018.02.038. Epub 2018 Feb 16.

Strawberry extracts efficiently counteract inflammatory stress induced by the endotoxin lipopolysaccharide in Human Dermal Fibroblast.

Author information

1
Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, 60131, Ancona, Italy.
2
Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, 60131, Ancona, Italy; Area de Nutrición y Salud, Universidad Internacional Iberoamericana (UNINI), Campeche, C.P.24040, Mexico.
3
Departamento de Quimica Analıtica y Alimentaria, Grupo de Nutricion y Bromatologıa, Universidade de Vigo, 32004 Ourense, Spain.
4
Departamento de Fisiologia, Instituto de Nutrición y Tecnología de los Alimentos ''José Mataix", Centro de Investigaciones Biomedicas, Universidad de Granada, 18100 Granada, Spain.
5
Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, 60131, Ancona, Italy.
6
Dipartimento di Scienze Biomediche e Sanità Pubblica, Facoltà di Medicina, Università Politecnica delle Marche Via Ranieri 65, 60131 Ancona, Italy.
7
Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, 60131, Ancona, Italy; Centre for Nutrition & Health, Universidad Europea del Atlantico (UEA), C/Isabel Torres 21, 39011 Santander, Spain. Electronic address: m.a.battino@univpm.it.

Abstract

A protracted pro-inflammatory state is the common denominator in the development, progression and complication of the common chronic diseases. Dietary antioxidants represent an efficient tool to counteract this inflammatory state. The aim of the present work was to evaluate the effects of strawberry extracts on inflammation evoked by E. Coli lipopolysaccharide in Human Dermal Fibroblast, by measuring reactive oxygen species production, apoptosis rate, antioxidant enzymes activity, mitochondria functionality and also investigating the molecular pathway involved in inflammatory and antioxidant response. The results demonstrated that strawberry pre-treatment reduced intracellular reactive oxygen species levels, apoptotic rate, improved antioxidant defences and mitochondria functionality in lipopolysaccharide -treated cells. Strawberry exerted these protective activities through the inhibition of the NF-kB signalling pathway and the stimulation of the Nrf2 pathway, with a mechanism AMPK-dependent. These results confirm the health benefits of strawberry in the prevention of inflammation and oxidative stress condition in lipopolysaccharide-treated cells.

KEYWORDS:

Antioxidant response; Human Dermal Fibroblast; Inflammation; LPS; Oxidative stress; Prevention; Strawberry

PMID:
29458159
DOI:
10.1016/j.fct.2018.02.038
[Indexed for MEDLINE]

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