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Carbohydr Polym. 2018 Apr 15;186:321-331. doi: 10.1016/j.carbpol.2018.01.075. Epub 2018 Jan 31.

Polysaccharide from Ostrea rivularis attenuates reproductive oxidative stress damage via activating Keap1-Nrf2/ARE pathway.

Author information

1
Mathematical Engineering Academy of Chinese Medicine, and School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, PR China.
2
Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an 223001, Jiangsu, PR China.
3
Jiangsu Key Laboratory of Regional Resource Exploitation and Medicinal Research, Huaiyin Institute of Technology, Huai'an 223003, Jiangsu, PR China.
4
Mathematical Engineering Academy of Chinese Medicine, and School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, PR China. Electronic address: qpxiong@gmail.com.
5
Mathematical Engineering Academy of Chinese Medicine, and School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, PR China. Electronic address: danyan64@gzucm.edu.cn.

Abstract

The purpose of this study was to investigate the effects of Ostrea rivularis polysaccharide (ORP) against testicular oxidative stress injury via kelch-like ECH-associated protein 1-nuclear erythroid 2-related factor 2/antioxidant response element (Keap1-Nrf2/ARE) pathway. In pharmacological experiments in vivo, ORP administration could dose-dependently inhibit body and testicular weight loss, ameliorate epididymal sperm quality and protect reproductive impairment in cyclophosphamide-induced male Balb/c mice. Moreover, the mechanism in vivo might be elucidated that ORP could increase expression level of Nrf2 and its downstream ARE gene battery in the testis, promote production of corresponding antioxidative enzymes and protein, and enhance Keap1-Nrf2/ARE signaling pathway to avoid male reproductive dysfunction. In addition, ORP treatment could improve survival capacity of H2O2-induced TM4 cells and its antioxidant mechanism in vitro also had been verified to activate Keap1-Nrf2/ARE signaling pathway. Overall, these results showed that ORP as a potent antioxidant could reduce reproductive oxidative stress damage related to Keap1-Nrf2/ARE pathway.

KEYWORDS:

Keap1-Nrf2/ARE pathway; Ostrea rivularis; Polysaccharide; Reproductive oxidative stress damage

PMID:
29455993
DOI:
10.1016/j.carbpol.2018.01.075
[Indexed for MEDLINE]

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