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J Nutr Biochem. 2018 Jun;56:55-64. doi: 10.1016/j.jnutbio.2018.01.005. Epub 2018 Jan 31.

Long-term treatment with green tea polyphenols modifies the gut microbiome of female sprague-dawley rats.

Author information

1
Interdisciplinary Toxicology Program, University of Georgia, Athens, GA 30602, USA; Department of Environmental Health Science, University of Georgia, Athens, GA 30602, USA.
2
Department of Environmental Health Science, University of Georgia, Athens, GA 30602, USA; School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China.
3
Department of Pathology, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.
4
Interdisciplinary Toxicology Program, University of Georgia, Athens, GA 30602, USA; Department of Environmental Health Science, University of Georgia, Athens, GA 30602, USA. Electronic address: jswang@uga.edu.

Abstract

Green tea polyphenols (GTP) have been shown to exert a spectrum of health benefits to animals and humans. It is plausible that the beneficial effects of GTP are a result of its interaction with the gut microbiota. This study evaluated the effect of long-term treatment with GTP on the gut microbiota of experimental rats and the potential linkage between changes of the gut microbiota with the beneficial effects of GTP. Six-month-old Sprague-Dawley rats were randomly allocated into three dosing regimens (0, 0.5%, and 1.5% of GTP) and followed for 6 months. At the end of month 3 or month 6, half of the animals from each group were sacrificed and their colon contents were collected for microbiome analysis using 16S ribosomal RNA and shotgun metagenomic community sequencing. GTP treatment significantly decreased the biodiversity and modified the microbial community in a dose-dependent manner; similar patterns were observed at both sampling times. Multiple operational taxonomic units and phylotypes were modified: the phylotypes Bacteroidetes and Oscillospira, previously linked to the lean phenotype in human and animal studies, were enriched; and Peptostreptococcaceae previously linked to colorectal cancer phenotype was depleted in GTP treated groups in a dose-dependent manner. Several microbial gene orthologs were modified, among which genes related to energy production and conversion were consistently enriched in samples from month 6 in a dose-dependent manner. This study showed that long-term treatment with GTP induced a dose-dependent modification of the gut microbiome in experimental rats, which might be linked to beneficial effects of GTP.

KEYWORDS:

16S gene survey; Catechin; Green Tea Polyphenol; Gut microbiome; Metagenomics; Next generation sequencing

PMID:
29454999
PMCID:
PMC6022747
[Available on 2019-06-01]
DOI:
10.1016/j.jnutbio.2018.01.005

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