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Food Chem Toxicol. 2018 Apr;114:180-189. doi: 10.1016/j.fct.2018.02.034. Epub 2018 Feb 15.

Inhibitory effects of selenium on cadmium-induced cytotoxicity in PC12 cells via regulating oxidative stress and apoptosis.

Author information

1
Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan.
2
Laboratory of Environmental Health Science, Faculty of Health Science, Hokkaido University, Sapporo 060-0812, Japan; Group of Environmental Adaptation Science, Faculty of Environmental Earth Science, Hokkaido University, Sapporo 060-0810, Japan; Department of Public Health and Informatics, Jahangirnagar University, Dhaka 1342, Bangladesh.
3
Laboratory of Environmental Health Science, Faculty of Health Science, Hokkaido University, Sapporo 060-0812, Japan.
4
Research Division of Higher Education, Institute for the Advancement of Higher Education, Hokkaido University, Sapporo 060-0817, Japan.
5
Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan; Group of Environmental Adaptation Science, Faculty of Environmental Earth Science, Hokkaido University, Sapporo 060-0810, Japan. Electronic address: kura@ees.hokudai.ac.jp.

Abstract

Purpose of this study is to investigate mechanism/s of cyto-protection by selenium (Na2SeO3; Se4+) against cadmium (CdCl2; Cd2+)-induced cytotoxicity using PC12 cells. In addition, Se (5, 10, 20 and 40 μM) and Cd (2.5, 5 and 10 μM)-induced cytotoxicity is determined. Cytotoxicity assays and western blot analyses confirmed that Se (≥10 μM) promotes autophagic cell death via inhibition of mTOR activation and p62 accumulation due to increase of cellular oxidative stress. On the other hand, co-presence of non-toxic Se (5 μM) and toxic Cd (5 μM) showed to increase cell viability, glutathione and glutathione peroxidase 1 (GPx1) levels, and to decrease DNA fragmentation and lactate dehydrogenase (LDH) activity compared to Cd-treated (5 μM) cells alone. Furthermore, western blot analyses of cytochrome c and ERK1 indicated that Cd-induced apoptotic cell death in PC12 cells. However, the co-exposure of Se with Cd significantly decreases the release of cytochrome c into cytosol from mitochondria, and up-regulates ERK1 protein to inhibit Cd-induced apoptosis. In conclusion, Se (≥10 μM) possess cytotoxicity in PC12 cells; however, co-presence of Se (5 μM) with Cd (5 μM) protects against Cd-induced apoptosis in PC12 cells due to inhibition of Cd-induced oxidative stress and subsequently suppression of mitochondrial apoptosis pathway.

KEYWORDS:

Apoptosis; Autophagy; Cytochrome c; Glutathione; Glutathione peroxidase; Oxidative stress

PMID:
29454865
DOI:
10.1016/j.fct.2018.02.034
[Indexed for MEDLINE]

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