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Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2425-2430. doi: 10.1073/pnas.1714246115. Epub 2018 Feb 16.

Select and resequence reveals relative fitness of bacteria in symbiotic and free-living environments.

Author information

1
Department of Plant and Microbial Biology, University of Minnesota, St. Paul, MN 55108.
2
BioTechnology Institute, University of Minnesota, St. Paul, MN 55108.
3
Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108.
4
Department of Soil, Water, and Climate, University of Minnesota, St. Paul, MN 55108.
5
Department of Plant and Microbial Biology, University of Minnesota, St. Paul, MN 55108; ptiffin@umn.edu.

Abstract

Assays to accurately estimate relative fitness of bacteria growing in multistrain communities can advance our understanding of how selection shapes diversity within a lineage. Here, we present a variant of the "evolve and resequence" approach both to estimate relative fitness and to identify genetic variants responsible for fitness variation of symbiotic bacteria in free-living and host environments. We demonstrate the utility of this approach by characterizing selection by two plant hosts and in two free-living environments (sterilized soil and liquid media) acting on synthetic communities of the facultatively symbiotic bacterium Ensifer meliloti We find (i) selection that hosts exert on rhizobial communities depends on competition among strains, (ii) selection is stronger inside hosts than in either free-living environment, and (iii) a positive host-dependent relationship between relative strain fitness in multistrain communities and host benefits provided by strains in single-strain experiments. The greatest changes in allele frequencies in response to plant hosts are in genes associated with motility, regulation of nitrogen fixation, and host/rhizobia signaling. The approach we present provides a powerful complement to experimental evolution and forward genetic screens for characterizing selection in bacterial populations, identifying gene function, and surveying the functional importance of naturally occurring genomic variation.

KEYWORDS:

Ensifer meliloti; Medicago; evolve and resequence; facultative mutualism; synthetic community

PMID:
29453274
PMCID:
PMC5877963
DOI:
10.1073/pnas.1714246115
[Indexed for MEDLINE]
Free PMC Article

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