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Am J Clin Pathol. 2018 Mar 7;149(4):344-351. doi: 10.1093/ajcp/aqx161.

Daily Biopsy Diagnosis in Surgical Pathology: Concordance Between Light Microscopy and Whole-Slide Imaging in Real-Life Conditions.

Author information

1
Département de Biologie et Pathologie Médicales, Service de Pathologie Morphologique, Villejuif, France.
2
Service de Biostatistique et d'Epidémiologie, Gustave Roussy Cancer Campus, Villejuif, France.
3
TRIBVN Healthcare, Chatillon, France.
4
Faculté de Médecine de Bicêtre, Université Paris Saclay, Université Paris Sud XI, Le Kremlin-Bicêtre, France.

Abstract

Objectives:

The current challenge for the various digital whole-slide imaging (WSI) systems is to be definitively validated for diagnostic purposes. We designed a concordance study between glass slide and digital slide diagnosis in real-life conditions, coupled with an ergonomic study.

Methods:

Three senior pathologists evaluated, first in glass slides and then in digital slides, 119 biopsy cases, including 749 slides, with 332 H&E saffron stains and 417 additional techniques, mainly immunohistochemistry.

Results:

All digital slides, including specially stained slides, were interpretable. Concordance between glass slides and digital slides was observed in 87.4% of cases. Minor discordances were observed in 12 (10.1%) cases and major discordances, with therapeutic impact, in three (2.5%), including one related to WSI. The satisfaction of participants was high and increased with time.

Conclusions:

Our study confirms the feasibility and accuracy of WSI diagnosis, even for cases having multiple samples and requiring special staining techniques, such as immunohistochemistry and in situ hybridization.

PMID:
29452345
DOI:
10.1093/ajcp/aqx161
[Indexed for MEDLINE]

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