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Virus Res. 2018 Mar 15;248:24-30. doi: 10.1016/j.virusres.2018.02.006. Epub 2018 Feb 13.

Screening of binding proteins that interact with Chinese sacbrood virus VP3 capsid protein in Apis cerana larvae cDNA library by the yeast two-hybrid method.

Author information

1
Institute of Life Sciences, Jinzhou Medical University, No. 40, Section 3 Songpo Road, Jinzhou, Liaoning Province, 121001, China; College of Veterinary Medicine, Northeast Agricultural University, No. 59, Xiangfang the public Hamaji timber Street, Harbin, Heilongjiang Province, 150030, China.
2
Institute of Life Sciences, Jinzhou Medical University, No. 40, Section 3 Songpo Road, Jinzhou, Liaoning Province, 121001, China.
3
College of Veterinary Medicine, Northeast Agricultural University, No. 59, Xiangfang the public Hamaji timber Street, Harbin, Heilongjiang Province, 150030, China.
4
Honeybee Research Institute, Chinese Academy of Agricultural Sciences, Xiangshan, Beijing 100093, China.
5
Institute of Life Sciences, Jinzhou Medical University, No. 40, Section 3 Songpo Road, Jinzhou, Liaoning Province, 121001, China. Electronic address: jzykdxmmx@126.com.

Abstract

Chinese sacbrood virus (CSBV) causes larval death and apiary collapse of Apis cerana. VP3 is a capsid protein of CSBV but its function is poorly understood. To determine the function of VP3 and screen for novel binding proteins that interact with VP3, we conducted yeast two-hybrid screening, glutathione S-transferase pull-down, and co-immunoprecipitation assays. Galectin (GAL) is a protein involved in immune regulation and host-pathogen interactions. The yeast two-hybrid screen implicated GAL as a major VP3-binding candidate. The assays showed that the VP3 interacted with GAL. Identification of these cellular targets and clarifying their contributions to the host-pathogen interaction may be useful for the development of novel therapeutic and prevention strategies against CSBV infection.

KEYWORDS:

CSBV; Capsid protein VP3; Galectin; Protein interaction; Yeast two-hybrid

PMID:
29452163
DOI:
10.1016/j.virusres.2018.02.006
[Indexed for MEDLINE]

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