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Biosci Rep. 2018 Jul 31;38(4). pii: BSR20171212. doi: 10.1042/BSR20171212. Print 2018 Aug 31.

Association of tumor necrosis factor-α (TNF-α) gene promoter polymorphisms with aggressive and chronic periodontitis in the eastern Indian population.

Author information

1
Department of Biotechnology, Centre for Genetic studies, School of Biotechnology and Biological Sciences, West Bengal University of Technology, BF-142, Sector I, Salt Lake City, Kolkata, West Bengal 70064, India.
2
Department of Periodontia, North Bengal Dental College, Siliguri, West Bengal, 734012, India.
3
Department of Zoology, Cytogenetics and Genomics Research Unit, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal 700019, India.
4
Department of Biotechnology, Centre for Genetic studies, School of Biotechnology and Biological Sciences, West Bengal University of Technology, BF-142, Sector I, Salt Lake City, Kolkata, West Bengal 70064, India skd.humgenet@gmail.com.

Abstract

Background: Periodontitis is a very common inflammatory oral disease. Tumor necrosis factor-α (TNF-α) is a cytokine that has been involved with the gingival tissue destruction and remodeling occurrence. We investigated the association of single nucleotide polymorphisms (SNPs) in TNF-α gene promoter region with the susceptibility of aggressive and chronic periodontitis in the eastern Indian population.Methods: A total of 397 DNA samples from venous blood were isolated. 40 individuals were aggressive periodontitis patients, 157 were identified chronic periodontitis patients, and the remaining 200 were healthy individuals. Five SNPs of TNF-α at promoter region (rs361525, rs1800629, rs1799724, rs1800630, and rs1799964) were genotyped by PCR-sequencing in periodontitis patients and control subjects.Results: rs1800629 (-308G/A) polymorphism was more frequent in both aggressive and chronic periodontitis patients compared with the control population, though the allele frequency was different only in aggressive periodontitis patients. On the other hand, both the genotypic and allelic variation of rs361525 (-238G/A) polymorphism were found significantly less frequently in aggressive and chronic periodontitis than in controls. The other polymorphisms like rs1799724 (-857C/T) and rs1799964 (-1031T/C) were significantly different between chronic periodontitis patients and control subjects.Conclusion: The findings suggest that the rs1800629 (-308G/A) polymorphism of TNF-α gene is associated with both aggressive and chronic periodontitis while rs1799724 (-857C/T) and rs1799964 (-1031T/C) polymorphisms of TNF-α gene is associated only with the increased susceptibility to chronic periodontitis.

KEYWORDS:

PCR-sequencing; Periodontitis; SNP; TNF-α; allele; genotype

PMID:
29449347
PMCID:
PMC6066656
DOI:
10.1042/BSR20171212
[Indexed for MEDLINE]
Free PMC Article

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