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Nutr Metab (Lond). 2018 Feb 6;15:12. doi: 10.1186/s12986-018-0250-6. eCollection 2018.

Curcumin and piperine supplementation of obese mice under caloric restriction modulates body fat and interleukin-1β.

Author information

1
1Vascular Biology Laboratory, Jean Mayer USDA-Human Nutrition Research Center on Aging, (HNRCA) at Tufts University, Boston, MA 02111 USA.
2
2Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-0845 Japan.
3
3Vitamin Metabolism Laboratory, Jean Mayer USDA-Human Nutrition Research Center on Aging, (HNRCA) at Tufts University, Boston, MA 02111 USA.
4
4Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, Florida, 33136 USA.
5
5Mass Spectrometry Laboratory, Jean Mayer USDA-Human Nutrition Research Center on Aging, (HNRCA) at Tufts University, Boston, MA 02111 USA.
6
6Energy Metabolism Laboratory, Jean Mayer USDA-Human Nutrition Research Center on Aging, (HNRCA) at Tufts University, Boston, MA 02111 USA.
7
7Food and Biotechnology Innovation Project, New Industry Creation Hatchery Center (NICHe), Tohoku University, Sendai, 980-8579 Japan.
8
8Food and Health Science Research Unit, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-0845 Japan.

Abstract

Background:

Dietary bioactive compounds capable of improving metabolic profiles would be of great value, especially for overweight individuals undergoing a caloric restriction (CR) regimen. Curcumin (Cur), a possible anti-obesity compound, and piperine (Pip), a plausible enhancer of Cur's bioavailability and efficacy, may be candidate agents for controlling body fat, metabolism and low grade inflammation.

Methods:

47 eight-week-old male C57BL/6 mice were fed a high fat diet (HFD) for 23 weeks to induce obesity. Then, mice were divided into 5 groups. Group 1 continued on HFD ad libitum. The other 4 groups underwent CR (reduced 10% HFD intake for 10 weeks, 20% for 20 weeks) with Cur, Pip, Cur + Pip or none of these. Percent body fat, plasma inflammatory markers associated with obesity (interferon (IFN)-γ, interleukin (IL)-10, IL-12 p70, IL-1β, IL-6 and KC/GRO), plasma Cur metabolites and liver telomere length were measured.

Results:

Compared to the other groups, obese mice who underwent CR and received Cur + Pip in their diet lost more fat and had significantly lower IL-1β and KC/GRO. Tandem mass spectrometry analysis of plasma from obese mice under CR showed no difference in Cur metabolite levels between groups supplemented with Cur alone or combined with Pip. However, plasma IL-1β levels were inversely correlated with curcumin glucuronide. Minor modulation of telomere length were observed.

Conclusions:

It is plausible that supplementing the high fat diet of CR mice with Cur + Pip may increase loss of body fat and suppresses HFD induced inflammation. Combination of Cur and Pip has potential to enhance CR effects for the prevention of metabolic syndrome.

KEYWORDS:

Caloric restriction; Curcumin; Glucuronide; High fat diet; Inflammation; Meso scale discovery; Metabolic syndrome; Obesity; Piperine; Tandem mass spectrometry

Conflict of interest statement

All conditions and handling of the animals in this study were reviewed and approved by the animal care and use committee of the Jean Mayer USDA Human Nutrition Research Center on Aging (HNRCA) at Tufts University and conducted according to the NIH guidelines for the care and use of laboratory animals.Not applicable.The authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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