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Mol Cell Proteomics. 2018 Nov;17(11):2229-2241. doi: 10.1074/mcp.RA117.000018. Epub 2018 Feb 14.

Protein Palmitoylation Plays an Important Role in Trichomonas vaginalis Adherence.

Author information

1
From the ‡Laboratorio de Parásitos Anaerobios, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico Chascomús (IIB-INTECH), CONICET-UNSAM, Chascomús B7130IWA, Argentina.
2
§Department of Biological Chemistry, University of California, Los Angeles, California, 90095-1489.
3
¶Laboratorio de Bioquímica de Parásitos, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico Chascomús (IIB-INTECH), CONICET-UNSAM, Chascomús B7130IWA, Argentina.
4
‖Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California, 90095-1489.
5
From the ‡Laboratorio de Parásitos Anaerobios, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico Chascomús (IIB-INTECH), CONICET-UNSAM, Chascomús B7130IWA, Argentina; ndemiguel@intech.gov.ar.

Abstract

The flagellated protozoan parasite Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted infection worldwide. As an obligate extracellular pathogen, adherence to epithelial cells is critical for parasite survival within the human host and a better understanding of this process is a prerequisite for the development of therapies to combat infection. In this sense, recent work has shown S-acylation as a key modification that regulates pathogenesis in different protozoan parasites. However, there are no reports indicating whether this post-translational modification is a mechanism operating in T. vaginalis In order to study the extent and function of S-acylation in T. vaginalis biology, we undertook a proteomic study to profile the full scope of S-acylated proteins in this parasite and reported the identification of 363 proteins involved in a variety of biological processes such as protein transport, pathogenesis related and signaling, among others. Importantly, treatment of parasites with the palmitoylation inhibitor 2-bromopalmitate causes a significant decrease in parasite: parasite aggregation as well as adherence to host cells suggesting that palmitoylation could be modifying proteins that are key regulators of Trichomonas vaginalis pathogenesis.

KEYWORDS:

Adherence; Cell adhesion; Host-Pathogen Interaction; Infectious disease; Microbiology; Palmitoylation; Parasite; Pathogens; Post-translational modifications; Protein Modification

PMID:
29444981
PMCID:
PMC6210223
[Available on 2019-11-01]
DOI:
10.1074/mcp.RA117.000018

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