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J Vis Exp. 2018 Jan 18;(131). doi: 10.3791/56709.

A Microcontroller Operated Device for the Generation of Liquid Extracts from Conventional Cigarette Smoke and Electronic Cigarette Aerosol.

Author information

1
Cell & Molecular Biology, Tulane University; wanders2@tulane.edu.
2
Cell & Molecular Biology, Tulane University.
3
Biomedical Engineering, Tulane University.
4
Cell & Molecular Biology & Ophthalmology, Tulane University.

Abstract

Electronic cigarettes are the most popular tobacco product among middle and high schoolers and are the most popular alternative tobacco product among adults. High quality, reproducible research on the consequences of electronic cigarette use is essential for understanding emerging public health concerns and crafting evidence based regulatory policy. While a growing number of papers discuss electronic cigarettes, there is little consistency in methods across groups and very little consensus on results. Here, we describe a programmable laboratory device that can be used to create extracts of conventional cigarette smoke and electronic cigarette aerosol. This protocol details instructions for the assembly and operation of said device, and demonstrates the use of the generated extract in two sample applications: an in vitro cell viability assay and gas-chromatography mass-spectrometry. This method provides a tool for making direct comparisons between conventional cigarettes and electronic cigarettes, and is an accessible entry point into electronic cigarette research.

PMID:
29443043
PMCID:
PMC5908656
[Available on 2020-01-18]
DOI:
10.3791/56709
[Indexed for MEDLINE]

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