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J Nanosci Nanotechnol. 2018 May 1;18(5):3095-3101. doi: 10.1166/jnn.2018.14697.

Aptamer-Immobilized Surface Plasmon Resonance Biosensor for Rapid and Sensitive Determination of Virulence Determinant.

Author information

1
School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju 28644, South Korea.
2
College of Veterinary Medicine, Western University of Health Sciences, 309 E Second Street, Pomona CA 91766, USA.
3
Department of Food Science and Biotechnology, Shin Ansan University, 135, Sinansandaehak-ro, Danwon-Gu, Ansan 425-792, South Korea.
4
Department of Bioprocess Engineering, Chonbuk National University, 567 Baekje-Daero, Deokjin-Gu Jeonju, Jeonbuk 54896, South Korea.

Abstract

Shigella sonnei isolate invasion plasmid antigen protein, IpaH, was successfully expressed in recombinant overexpression bacterial system. The soluble expression IpaH was enhanced with molecular chaperon co-expressed environment. Specific aptamer IpaH17 was isolated through the SELEX process and showed fM binding affinity. IpaH17-SPR biosensor platform was involved to verify the binding sensitivity and specificity. The IpaH concentration dependent IpaH17-SPR sensor response was highly linear with a linear regression constant of 99.4% in the range between 0 and 100 ng/mL. In addition, S. sonnei revealed the specific RU value and detected in a real-time manner within 1 hour. Our study indicated that IpaH17-SPR sensor can allow for rapid, sensitive and specific determination of Shigella sonnei virulent factor.

PMID:
29442808
DOI:
10.1166/jnn.2018.14697
[Indexed for MEDLINE]

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