Send to

Choose Destination
J Nanosci Nanotechnol. 2018 May 1;18(5):3095-3101. doi: 10.1166/jnn.2018.14697.

Aptamer-Immobilized Surface Plasmon Resonance Biosensor for Rapid and Sensitive Determination of Virulence Determinant.

Author information

School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju 28644, South Korea.
College of Veterinary Medicine, Western University of Health Sciences, 309 E Second Street, Pomona CA 91766, USA.
Department of Food Science and Biotechnology, Shin Ansan University, 135, Sinansandaehak-ro, Danwon-Gu, Ansan 425-792, South Korea.
Department of Bioprocess Engineering, Chonbuk National University, 567 Baekje-Daero, Deokjin-Gu Jeonju, Jeonbuk 54896, South Korea.


Shigella sonnei isolate invasion plasmid antigen protein, IpaH, was successfully expressed in recombinant overexpression bacterial system. The soluble expression IpaH was enhanced with molecular chaperon co-expressed environment. Specific aptamer IpaH17 was isolated through the SELEX process and showed fM binding affinity. IpaH17-SPR biosensor platform was involved to verify the binding sensitivity and specificity. The IpaH concentration dependent IpaH17-SPR sensor response was highly linear with a linear regression constant of 99.4% in the range between 0 and 100 ng/mL. In addition, S. sonnei revealed the specific RU value and detected in a real-time manner within 1 hour. Our study indicated that IpaH17-SPR sensor can allow for rapid, sensitive and specific determination of Shigella sonnei virulent factor.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Ingenta plc
Loading ...
Support Center