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Sci Rep. 2018 Feb 13;8(1):2919. doi: 10.1038/s41598-018-21153-y.

Combined aptamer and transcriptome sequencing of single cells.

Author information

1
Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, 94158, California, USA.
2
Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, 94158, California, USA. adam@abatelab.org.
3
California Institute for Quantitative Biosciences, University of California San Francisco, San Francisco, 94158, California, USA. adam@abatelab.org.
4
Chan Zuckerberg Biohub, San Francisco, 94158, California, USA. adam@abatelab.org.

Abstract

The transcriptome and proteome encode distinct information that is important for characterizing heterogeneous biological systems. We demonstrate a method to simultaneously characterize the transcriptomes and proteomes of single cells at high throughput using aptamer probes and droplet-based single cell sequencing. With our method, we differentiate distinct cell types based on aptamer surface binding and gene expression patterns. Aptamers provide advantages over antibodies for single cell protein characterization, including rapid, in vitro, and high-purity generation via SELEX, and the ability to amplify and detect them with PCR and sequencing.

PMID:
29440771
PMCID:
PMC5811598
DOI:
10.1038/s41598-018-21153-y
[Indexed for MEDLINE]
Free PMC Article

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