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Appl Environ Microbiol. 2018 Feb 9. pii: AEM.00044-18. doi: 10.1128/AEM.00044-18. [Epub ahead of print]

Deposition of Bacteria and Bacterial Spores by Bathroom Hot Air Hand Dryers.

Author information

1
Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT 06030-3305.
2
Department of Pathology and Laboratory Medicine, Hartford Hospital, Hartford, CT 06102.
3
Department of Pathology and Laboratory Medicine, UConn Health, Farmington, CT 06030.
4
Department of Medical Sciences, Frank H Netter MD School of Medicine, Quinnipiac University, North Haven CT 06473.
5
Department of Pathology and Laboratory Medicine, UConn Health, Farmington, CT 06030 setlow@nso2.uchc.edu tmurray@connecticutchildrens.org.
6
Division of Infectious Diseases and Immunology, Connecticut Children's Medical Center, Hartford, CT 06518.
7
Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT 06030-3305 setlow@nso2.uchc.edu tmurray@connecticutchildrens.org.

Abstract

Hot air hand dryers in multiple men's and women's bathrooms in 3 basic science research areas in an academic health center were screened for their deposition on plates of: i) total bacteria, some of which were identified; and ii) a kanamycin resistant Bacillus subtilis strain, PS533, spores of which are produced in large amounts in one basic science research laboratory. Plates exposed to hand dryer air for 30 seconds averaged 18-60 colonies/plate but interior hand dryer nozzle surfaces had minimal bacterial levels, plates exposed to bathroom air for 2 minutes with hand dryers off averaged ≤1 colony, and plates exposed to bathroom air moved by a small fan for 20 minutes had averages of 15 and 12 colonies/plate in two buildings tested. Retrofitting hand dryers with HEPA filters reduced bacterial deposition by hand dryers ∼4-fold, and potential human pathogens were recovered from plates exposed to hand dryer air whether or not a HEPA filter was present, and from bathroom air moved by a small fan. Spore-forming colonies, identified as B. subtilis PS533 averaged ∼2.5-5% of bacteria deposited by hand dryers throughout basic research areas examined regardless of distance from the spore forming laboratory, and these were almost certainly deposited as spores. Comparable results were obtained when bathroom air was sampled for spores. These results indicate that many kinds of bacteria, including potential pathogens and spores, can be deposited on hands exposed to bathroom hand dryers, and that spores could be dispersed throughout buildings and deposited on hands by hand dryers.Importance While there is evidence that bathroom hand dryers can disperse bacteria from hands or deposit bacteria on surfaces, including recently washed hands, there is less information on: i) the organisms dispersed by hand dryers; ii) if hand dryers provide a reservoir of bacteria or simply blow large amounts of bacterially contaminated air; and iii) if bacterial spores are deposited on surfaces by hand dryers. Consequently, this study has implications for the control of opportunistic bacterial pathogens and spores in public environments including healthcare settings. Within a large building, potentially pathogenic bacteria including bacterial spores may travel between rooms, and subsequent bacterial/spore deposition by hand dryers is a possible mechanism for spread of infectious bacteria including spores of potential pathogens if present.

PMID:
29439992
PMCID:
PMC5881072
[Available on 2018-10-02]
DOI:
10.1128/AEM.00044-18

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