Induction of systemic inflammation by hyaluronan and hsp70 in women with pre-eclampsia

Cytokine. 2018 May:105:23-31. doi: 10.1016/j.cyto.2018.02.007. Epub 2018 Feb 10.

Abstract

Pre-eclampsia (PE) is a human pregnancy syndrome with abnormal activation of the innate immune response. The study evaluated the involvement of molecular structures called damage-associated molecular patterns (DAMPs), such as hyaluronan (HA) and heat shock proteins (Hsp) on NLRP1 and NLRP3 inflammasomes activation in peripheral blood monocytes. Twenty pre-eclamptic women, 20 normotensive pregnant women (NT) and 20 non-pregnant women (NP) were studied. Enzyme immunoassay was employed for the determination of HA, Hsp70 and High mobility group Box 1 (HMGB1) in plasma, as well as for the detection of Interleukin-1β (IL-1β), IL-18 and tumor necrosis factor alpha (TNF-α) in the supernatant of monocytes cultured with or without HA and Hsp70. The inflammasomes induction was evaluated by the quantification of mRNA for NLRP1, NLRP3, caspase-1, IL-1β, IL-18, HMGB1 and TNF-α by qPCR in monocyte culture. The results showed significantly higher plasma levels of HA, Hsp70 and HMGB1 in pre-eclamptic women than in NT and NP women. Monocytes from women with PE showed endogenous activation of NLRP1 and NLRP3 inflammasomes, and expressed high amounts of IL-1β, IL-18, HMGB1 and TNF-α. The stimulation of monocytes with HA increased the gene expression of NLRP1, NLRP3, caspase-1, TNF-α, IL-1β, HMGB1 and IL-18 and the production of IL-1β in pre-eclamptic women. Monocytes cultured with Hsp70 produced elevated levels of IL-1β and TNF-α through a mechanism independent of inflammasomes activation. These results suggest the participation of these DAMPs in the systemic inflammatory response that is characteristic of PE.

Keywords: Cytokines; DAMPs; Inflammasomes; Monocytes; Pre-eclampsia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Cytokines / biosynthesis
  • Cytokines / genetics
  • Female
  • Gene Expression Regulation
  • HSP70 Heat-Shock Proteins / metabolism*
  • Humans
  • Hyaluronic Acid / metabolism*
  • Inflammation / blood
  • Inflammation / pathology*
  • Monocytes / metabolism
  • Pathogen-Associated Molecular Pattern Molecules / blood
  • Pre-Eclampsia / blood
  • Pre-Eclampsia / metabolism*
  • Pregnancy
  • Young Adult

Substances

  • Cytokines
  • HSP70 Heat-Shock Proteins
  • Pathogen-Associated Molecular Pattern Molecules
  • Hyaluronic Acid