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Int J Mol Sci. 2018 Feb 13;19(2). pii: E567. doi: 10.3390/ijms19020567.

Molecular-Assisted Pollen Grain Analysis Reveals Spatiotemporal Origin of Long-Distance Migrants of a Noctuid Moth.

Author information

1
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops and Fujian Province Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Hong.Chang.PhD@gmail.com.
2
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Hong.Chang.PhD@gmail.com.
3
College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China. jianglongguo88@163.com.
4
Department of Plant Protection, Henan Institute of Science and Technology, Xinxiang 453003, China. fxw1983@126.com.
5
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. lyq364467268@163.com.
6
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. kagwyckhuys@gmail.com.
7
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops and Fujian Province Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. ymhou@fafu.edu.cn.
8
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. wukongming@caas.cn.

Abstract

Pollen grains are regularly used as markers to determine an insect's movement patterns or host (plant) feeding behavior, yet conventional morphology-based pollen grain analysis (or palynology) encounters a number of important limitations. In the present study, we combine conventional analytical approaches with DNA meta-barcoding to identify pollen grains attached to migrating adults of the turnip moth, Agrotis segetum (Lepidoptera: Noctuidae) in Northeast China. More specifically, pollen grains were dislodged from 2566 A. segetum long-distance migrants captured on Beihuang Island (Bohai Sea) and identified to many (plant) species level. Pollen belonged to 26 families of plants, including Fagaceae, Oleaceae, Leguminosae, Asteraceae, Pinaceae and Rosaceae, including common species such as Citrus sinensis, Olea europaea, Ligustrum lucidum, Robinia pseudoacacia, Castanopsis echinocarpa, Melia azedarach and Castanea henryi. As the above plants are indigenous to southern climes, we deduce that A. segetum forage on plants in those locales prior to engaging in northward spring migration. Our work validates the use of DNA-assisted approaches in lepidopteran pollination ecology research and provides unique and valuable information on the adult feeding range and geographical origin of A. segetum. Our findings also enable targeted (area-wide) pest management interventions or guide the future isolation of volatile attractants.

KEYWORDS:

Agrotis segetum; DNA barcoding; aerobiology; migration; plant-pollinator; pollen grain analysis

PMID:
29438348
PMCID:
PMC5855789
DOI:
10.3390/ijms19020567
[Indexed for MEDLINE]
Free PMC Article

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