Prosomes exist in plant cells too

A Kremp; M Schliephacke; U Kull; H P Schmid

doi:10.1016/0014-4827(86)90500-8

Prosomes exist in plant cells too

Exp Cell Res. 1986 Oct;166(2):553-7. doi: 10.1016/0014-4827(86)90500-8.

Authors

A Kremp, M Schliephacke, U Kull, H P Schmid

PMID: 2943602
DOI: 10.1016/0014-4827(86)90500-8

Abstract

A 19S particle was purified from tobacco (Nicotiana rustica) leaf cells. Its density was determined as 1296 g/cm3 in Cs2SO4-DMSO gradients, indicating the presence of RNA and protein. Polyacrylamide gel electrophoresis (PAGE) revealed eight distinct proteins in the range of 20-30 kD and RNA in the range of 70-80 nucleotides. Electron microscopic examination showed the same raspberry-shaped structure with a central depression as described for prosomes. We conclude that tobacco 19S particles represent small cytoplasmic complexes, possessing biochemical and structural characteristics similar to the hither-to known prosomes of animal cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Centrifugation, Density Gradient
Microscopy, Electron
Molecular Weight
Nicotiana
Plant Proteins / analysis
Plants / analysis*
Plants / ultrastructure
Plants, Toxic
Ribonucleoproteins / analysis*
Ribonucleoproteins, Small Nuclear

Substances

Plant Proteins
Ribonucleoproteins
Ribonucleoproteins, Small Nuclear