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J Nat Med. 2006 Jul;60(3):217-224. doi: 10.1007/s11418-006-0041-7. Epub 2006 May 24.

Inhibitory effect of Yunnan traditional medicines on hepatitis C viral polymerase.

Author information

1
Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan.
2
Faculty of Pharmaceutical Sciences, Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, Japan.
3
Kunming Institute of Botany, The Chinese Academy of Sciences, 650204, Kunming, People's Republic of China.
4
Kyoritsu University of Pharmacy, 1-5-30 Shibakoen, Minato-ku, Tokyo, 105-8512, Japan.
5
Department of Viral Oncology, Institute for Virus Research, Kyoto University, Sakyo-ku, Kyoto, 606-8507, Japan.
6
Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan. saibo421@ms.toyama-mpu.ac.jp.

Abstract

For the purpose of developing novel anti-hepatitis C virus (HCV) agents from natural resources, 93 Yunnan crude drugs were screened for their inhibitory effects on RNA-dependent RNA polymerase (RdRp) of HCV. Although 71 methanol extracts and 50 water extracts inhibited HCV-RdRp by more than 50% at a concentration of 50 μg/ml, the majority of them contained a high percentage of tannins. However, methanol extracts of Plumbago zeylanica (branch), Maytenus fookerii (leaf) and Huashidancha (Y61, branch and leaf), and water extracts of Potentilla griffithii (whole plant) and Salvia yunnanensis (underground part), having IC50 values of less than 10 μg/ml, showed less than 10% tannin content. In addition, from a methanol extract of Tripterygium hypoglaucum (root bark), demethylzeylasteral was isolated as a strongly inhibitory substance against HCV-RdRp.

KEYWORDS:

Demethylzeylasteral; Hepatitis C virus; RNA-dependent RNA polymerase; Tripterygium hypoglaucum; Yunnan crude drugs

PMID:
29435894
DOI:
10.1007/s11418-006-0041-7

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