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FEBS Open Bio. 2017 Dec 27;8(2):158-167. doi: 10.1002/2211-5463.12302. eCollection 2018 Feb.

The Stl repressor from Staphylococcus aureus is an efficient inhibitor of the eukaryotic fruitfly dUTPase.

Author information

1
Institute of Enzymology Research Centre for Natural Sciences Hungarian Academy of Sciences Budapest Hungary.
2
Department of Applied Biotechnology Budapest University of Technology and Economics Hungary.
3
Institute of Organic Chemistry Research Centre for Natural Sciences Hungarian Academy of Sciences Budapest Hungary.

Abstract

DNA metabolism and repair is vital for the maintenance of genome integrity. Specific proteinaceous inhibitors of key factors in this process have high potential for deciphering pathways of DNA metabolism and repair. The dUTPase enzyme family is responsible for guarding against erroneous uracil incorporation into DNA. Here, we investigate whether the staphylococcal Stl repressor may interact with not only bacterial but also eukaryotic dUTPase. We provide experimental evidence for the formation of a strong complex between Stl and Drosophila melanogaster dUTPase. We also find that dUTPase activity is strongly diminished in this complex. Our results suggest that the dUTPase protein sequences involved in binding to Stl are at least partially conserved through evolution from bacteria to eukaryotes.

KEYWORDS:

Stl; dUTPase; inhibition

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