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J Clin Virol. 2018 Apr;101:69-73. doi: 10.1016/j.jcv.2018.02.002. Epub 2018 Feb 6.

Self-sampling with HPV mRNA analyses from vagina and urine compared with cervical samples.

Author information

1
Department of Obstetrics and Gynecology, Skåne University Hospital, Lund, Lund University, Sweden.
2
Department of Clinical Microbiology, Skåne University Hospital, Lund, Lund University, Sweden.
3
Department of Obstetrics and Gynecology, Skåne University Hospital, Lund, Lund University, Sweden. Electronic address: christer.borgfeldt@med.lu.se.

Abstract

BACKGROUND:

In order to increase coverage in the organized cervical screening program, self-sampling with HPV analyses has been suggested.

OBJECTIVES:

The aim was to compare human papillomavirus (HPV) mRNA detection in vaginal and urine self-collected samples with clinician-taken cervical samples and the corresponding clinician-taken histological specimens.

STUDY DESIGN:

Self-collected vaginal, urine and clinician-taken cervical samples were analyzed from 209 women with the Aptima mRNA assay (Hologic Inc, MA, USA). Cervical cytology, colposcopy, biopsy and/or the loop electrosurgical excision procedure (LEEP) were performed in every examination.

RESULTS:

The sensitivity of the HPV mRNA test in detecting high-grade squamous intraepithelial lesions (HSIL)/adenocarcinoma in situ (AIS)/cancer cases was as follows: for the vaginal self-samples 85.5% (95% CI; 75.0-92.8), the urinary samples 44.8% (95% CI; 32.6-57.4), and for routine cytology 81.7% (95% CI; 70.7-89.9). For the clinician-taken cervical HPV samples the sensitivity of the HPV mRNA test in detecting HSIL/AIS/cancer was 100.0% (95% CI; 94.9-100.0). The specificity of the HPV mRNA was similar for the clinician-taken cervical HPV samples and the self-samples: 49.0% vs. 48.1%. The urinary HPV samples had a specificity of 61.9% and cytology had a specificity of 93.3%.

CONCLUSION:

The sensitivity of the Aptima HPV mRNA test in detecting HSIL/AIS/cancer from vaginal self-samples was similar to that of routine cytology. The Aptima HPV mRNA vaginal self-sampling analysis may serve as a complement in screening programs.

KEYWORDS:

HPV analyses; HPV mRNA; Self-collected vaginal smear; Urinary sample

PMID:
29433016
DOI:
10.1016/j.jcv.2018.02.002
[Indexed for MEDLINE]

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