Format

Send to

Choose Destination
J Ethnopharmacol. 2018 May 10;217:89-97. doi: 10.1016/j.jep.2018.02.011. Epub 2018 Feb 10.

Suppression of lung inflammation by the methanol extract of Spilanthes acmella Murray is related to differential regulation of NF-κB and Nrf2.

Author information

1
School of Korean Medicine, Pusan National University, Yangsan 50612, Republic of Korea.
2
Institute of Ecology and Biological Resources (IEBR), 18 Hoang Quoc Viet, Cay Giay, Ha Noi, Vietnam.
3
Korea Research Institute of Bioscience & Biotechnology, Daejeon 34141, Republic of Korea.
4
School of Korean Medicine, Pusan National University, Yangsan 50612, Republic of Korea; Department of Internal Medicine, Korean Medicine Hospital, Pusan National University, Yangsan 50612, Republic of Korea.
5
Laboratory of Molecular and Pharmacological Cell Biology, College of Pharmacy, Chung-Ang University, Seoul 06974, Republic of Korea.
6
Jeonju AgroBio-Materials Institute, Jeonju 57810, Republic of Korea.
7
School of Korean Medicine, Pusan National University, Yangsan 50612, Republic of Korea. Electronic address: mjoo@pusan.ac.kr.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Although Spilanthes acmella has been used to relieve inflammation, fever, pain, or infection in traditional Asian medicine, experimental evidence supporting these functions is scarce. Here, we examined an anti-inflammatory function and a possible underlying mechanism of S. acmella Murray (SAM).

MATERIALS AND METHOD:

The methanol extract of SAM was fingerprinted by HPLC. C57BL/6 mice were administered with a single intratracheal (i.t.) LPS and 2 h later with a single i.t. SAM. The effect of SAM on lung inflammation was assessed by histology, semi-quantitative RT-PCR, and MPO assay of lung tissue. The effects of SAM on a pro-inflammatory factor NF-κB and an anti-inflammatory factor Nrf2 were analyzed by immunoblotting of nuclear proteins and by semi-quantitative RT-PCR analysis of mRNA of the genes governed by these transcription factors. V5-Nrf2 was precipitated by an anti-V5 antibody and the ubiquitinated V5-Nrf2 was revealed by immunoblotting of HA-tagged ubiquitin.

RESULTS:

The i.t. SAM robustly diminished a neutrophilic lung inflammation induced by i.t. LPS treatment of mice. In RAW 264.7 cells, SAM suppressed the nuclear localization of NF-κB and the expression of NF-κB-dependent cytokine genes. SAM increased the level of Nrf2 in the nucleus and the expression of Nrf2-dependent genes while suppressing ubiquitination of Nrf2.

CONCLUSION:

Our results suggest that SAM can suppress a neutrophilic inflammation in mouse lungs, which is associated with suppressed NF-κB and activated Nrf2. Our results provide experimental evidence supporting the anti-inflammatory function of S. acmella.

KEYWORDS:

Anti-inflammation; NF-B; Neutrophilic lung inflammation; Nrf2; Spilanthes acmella Murray

PMID:
29432855
DOI:
10.1016/j.jep.2018.02.011
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center