Cultivation of multiple genera of hydrogenotrophic methanogens from different environmental niches

Akshay Joshi; Vikram Lanjekar; Prashant K Dhakephalkar; Sumit Singh Dagar

doi:10.1016/j.anaerobe.2018.02.001

Cultivation of multiple genera of hydrogenotrophic methanogens from different environmental niches

Anaerobe. 2018 Apr:50:64-68. doi: 10.1016/j.anaerobe.2018.02.001. Epub 2018 Feb 9.

Authors

Akshay Joshi¹, Vikram Lanjekar¹, Prashant K Dhakephalkar¹, Sumit Singh Dagar²

Affiliations

¹ Bioenergy Group, Agharkar Research Institute, Pune, India.
² Bioenergy Group, Agharkar Research Institute, Pune, India. Electronic address: ssdagar@aripune.org.

PMID: 29432849
DOI: 10.1016/j.anaerobe.2018.02.001

Abstract

Six genera of hydrogenotrophic methanogens, namely Methanobrevibacter, Methanobacterium, Methanocorpusculum, Methanothermobacter, Methanoculleus, and Methanospirillum were cultivated from diverse environmental niches like rumen, feces, gut, and sediments using BY medium. We also report a putative novel genus and two novel species of methanogens isolated from termite, Indian star tortoise, and green iguana.

Keywords: Anaerobes; Archaea; Gut; Hot spring; Methane; Rumen.

MeSH terms

Animals
Environmental Microbiology*
Feces / microbiology
Geologic Sediments / microbiology
Hydrogen / metabolism*
Metagenome
Metagenomics / methods
Methane / metabolism*
Microbiota*
Phylogeny
RNA, Ribosomal, 16S
Rumen / microbiology

Substances

RNA, Ribosomal, 16S
Hydrogen
Methane