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Elife. 2018 Feb 9;7. pii: e27454. doi: 10.7554/eLife.27454.

Global morphogenetic flow is accurately predicted by the spatial distribution of myosin motors.

Author information

1
Kavli Institute of Theoretical Physics, University of California, Santa Barbara, United States.
2
Department of Physics, University of California, Santa Barbara, United States.
3
Department of Molecular Biology, Princeton University, Princeton, United States.
4
Howard Hughes Medical Institute, Princeton University, Princeton, United States.

Abstract

During embryogenesis tissue layers undergo morphogenetic flow rearranging and folding into specific shapes. While developmental biology has identified key genes and local cellular processes, global coordination of tissue remodeling at the organ scale remains unclear. Here, we combine in toto light-sheet microscopy of the Drosophila embryo with quantitative analysis and physical modeling to relate cellular flow with the patterns of force generation during the gastrulation process. We find that the complex spatio-temporal flow pattern can be predicted from the measured meso-scale myosin density and anisotropy using a simple, effective viscous model of the tissue, achieving close to 90% accuracy with one time dependent and two constant parameters. Our analysis uncovers the importance of a) spatial modulation of myosin distribution on the scale of the embryo and b) the non-locality of its effect due to mechanical interaction of cells, demonstrating the need for the global perspective in the study of morphogenetic flow.

KEYWORDS:

D. melanogaster; biophysics; computational biology; morphogenesis; systems biology; tissue mechanics

PMID:
29424685
PMCID:
PMC5843464
DOI:
10.7554/eLife.27454
[Indexed for MEDLINE]
Free PMC Article

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