Format

Send to

Choose Destination
An Acad Bras Cienc. 2018 Jan-Mar;90(1):73-84. doi: 10.1590/0001-3765201820160131. Epub 2018 Feb 1.

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis.

Author information

1
Programa de Pós-Graduação em Microbiologia Agrícola e do Ambiente, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul/UFRGS, Rua Sarmento Leite, 500, 90050-170 Porto Alegre, RS, Brazil.
2
Instituto de Ciência e Tecnologia de Alimentos, Laboratório de Bioquímica e Microbiologia Aplicada, Universidade Federal do Rio Grande do Sul/UFRGS, Avenida Bento Gonçalves, 9500, 91501-970 Porto Alegre, RS, Brazil.

Abstract

The adhesion ability of bacteria to abiotic surfaces has important implications in food industries, because these organisms can survive for long periods through the biofilm formation. They can be transferred from one place to another in the industry causing contamination of the food processing environment. In this study, the antibacterial and antibiofilm activities of the antimicrobial peptide P34, characterized as a bacteriocin-like substance (BLS P34) were tested against planktonic and sessile cells of Staphylococcus aureus and Enterococcus faecalis isolated from foods. The BLS P34 showed inhibitory effect against all planktonic cells of E. faecalis. The inhibition of biofilm formation and the eradication of pre-formed biofilm were evaluated with the crystal violet assay and with the reduction of 3-bromide [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium. The BLS P34 promoted a reduction of percentage of adhered microbial cells on the surface, not being able to perform the complete elimination of biofilm formation. The metabolic activity of S. aureus biofilms decreased considerably between 41-95%. However, E. faecalis cells showed up metabolically stimulated. The BLS P34 has the potential antibiofilm for the species S. aureus. Studies suggest more detailed approaches to a better understanding of the interactions between the antimicrobial and bacterial cells within the biofilm structure.

PMID:
29424388
DOI:
10.1590/0001-3765201820160131
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Scientific Electronic Library Online
Loading ...
Support Center