Rapid IFM Dissection for Visualizing Fluorescently Tagged Sarcomeric Proteins

Bio Protoc. 2017 Nov 20;7(22):e2606. doi: 10.21769/BioProtoc.2606.

Abstract

Sarcomeres, the smallest contractile unit of muscles, are arguably the most impressive actomyosin structure. Yet a complete understanding of sarcomere formation and maintenance is missing. The Drosophila indirect flight muscle (IFM) has proven to be a very valuable model to study sarcomeres. Here, we present a protocol for the rapid dissection of IFM and analysis of sarcomeres using fluorescently tagged proteins.

Keywords: Dissection; Drosophila; GFP; Indirect flight muscle; Sarcomere; Z-disc.