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Regen Biomater. 2018 Feb;5(1):25-34. doi: 10.1093/rb/rbx030. Epub 2017 Dec 13.

A high-throughput study on endothelial cell adhesion and growth mediated by adsorbed serum protein via signaling pathway PCR array.

Author information

1
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China.
2
Nanjing Drum Tower Hospital, Nanjing 210008, P.R. China.
3
SQ Medical Device Co., Ltd., Nanjing 210008, P.R. China.
4
Shenzhen Research Institute, Peking University, Shenzhen 518055, P.R. China and.
5
R&D Center of Lifetech Scientific (Shenzhen) Co., Ltd, Shenzhen 518057, P.R. China.

Abstract

The purpose of this paper is to utilize the signaling pathway polymerase chain reaction (PCR) arrays to investigate the activation of two important biological signaling pathways in endothelial cell adhesion and growth mediated by adsorbed serum protein on the surface of bare and titanium nitride (TiN)-coated nickel titanium (NiTi) alloys. First, the endothelial cells were cultured on the bare and TiN-coated NiTi alloys and chitosan films as control for 4 h and 24 h, respectively. Then, the total RNA of the cells was collected and the PCR arrays were performed. After that, the differentially expressed genes in the transforming growth factor beta (TGF-β) signaling pathway and the regulation of actin cytoskeleton pathway were screened out; and the further bioinformatics analyses were performed. The results showed that both TGF-β signaling pathway and regulation of actin cytoskeleton pathway were activated in the cells after 4 h and 24 h culturing on the surface of bare and TiN-coated NiTi alloys compared to the chitosan group. The activated TGF-β signaling pathway promoted cell adhesion; the activated regulation of actin cytoskeleton pathway promoted cell adhesion, spreading, growth and motility. In addition, the activation of both pathways was much stronger in the cells cultured for 24 h versus 4 h, which indicated that cell adhesion and growth became more favorable with longer time on the surface of two NiTi alloy materials.

KEYWORDS:

biological signaling pathway; biomaterials; protein adsorption and cell adhesion; signaling pathway PCR array

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