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Nat Commun. 2018 Feb 8;9(1):587. doi: 10.1038/s41467-017-02284-8.

Pof8 is a La-related protein and a constitutive component of telomerase in fission yeast.

Author information

1
Stowers Institute for Medical Research, Kansas City, MO, 64110, USA.
2
RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, 01605, USA.
3
Stowers Institute for Medical Research, Kansas City, MO, 64110, USA. peter@baumannlab.org.
4
Howard Hughes Medical Institute, Kansas City, MO, 64110, USA. peter@baumannlab.org.
5
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, MO, 66160, USA. peter@baumannlab.org.
6
Institute of Developmental Biology and Neurobiology, Johannes Gutenberg University, 55099, Mainz, Germany. peter@baumannlab.org.

Abstract

Telomerase reverse transcriptase (TERT) and the non-coding telomerase RNA subunit (TR) constitute the core of telomerase. Here we now report that the putative F-box protein Pof8 is also a constitutive component of active telomerase in fission yeast. Pof8 functions in a hierarchical assembly pathway by promoting the binding of the Lsm2-8 complex to telomerase RNA, which in turn promotes binding of the catalytic subunit. Loss of Pof8 reduces TER1 stability, causes a severe assembly defect, and results in critically short telomeres. Structure profile searches identified similarities between Pof8 and telomerase subunits from ciliated protozoa, making Pof8 next to TERT the most widely conserved telomerase subunits identified to date.

PMID:
29422664
PMCID:
PMC5805746
DOI:
10.1038/s41467-017-02284-8
[Indexed for MEDLINE]
Free PMC Article

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