Format

Send to

Choose Destination
Virus Res. 2018 Mar 2;247:21-25. doi: 10.1016/j.virusres.2018.01.012. Epub 2018 Feb 3.

Molecular characterization of complete genome of a canine distemper virus associated with fatal infection in dogs in Gabon, Central Africa.

Author information

1
Centre International de Recherches Médicales de Franceville (CIRMF), Gabon; Institut National Supérieur d'Agronomie et de Biotechnologies (INSAB), Université des Sciences et Techniques de Masuku (USTM), Franceville, Gabon. Electronic address: gael_maganga@yahoo.fr.
2
Centre International de Recherches Médicales de Franceville (CIRMF), Gabon.
3
Ministère de l'agriculture et de l'élevage, Libreville, Gabon.
4
Centre International de Recherches Médicales de Franceville (CIRMF), Gabon; Institut de Recherche pour le Développement, UMR 224 (MIVEGEC), IRD/CNRS/UM1, Montpellier, France.
5
Centre International de Recherches Médicales de Franceville (CIRMF), Gabon; Centre National de Recherche Scientifique (CNRS) UMR3569, 25 rue du docteur Roux, Paris, France; Institut Pasteur, Unité Environnement et risques infectieux, Cellule d'Intervention Biologique d'Urgence, 25 rue du Docteur Roux, Paris, France.

Abstract

Canine distemper (CD) is the most deadly disease in dogs with mortality rates reaching 50%. The pathological agent, the CD virus (CDV), generally causes a severe systemic disease, although the nervous form can coexist with the acute catarrhal form in the same individual. In this study, we describe an outbreak of 18 cases of CD that occurred in 2015 in a German Shepherd dog population in northwestern Gabon. In addition, we determined the sequence of the CDV genotype associated with this fatal distemper infection in Gabon and compared it with other published CDV sequences. The CDV was detected using RT-PCR on cDNA from RNA of harvested brains and other organs. The identification was confirmed by sequencing amplicons. Moreover, we obtained the whole genome sequence using high-throughput sequencing. Phylogenetic analysis revealed that Gabonese CDV strain clustered with European strains belonging to the Europe genotype. This study provided the first molecular detection of the CDV strain associated with this fatal distemper infection in Central Africa region.

KEYWORDS:

CDV; Central Africa; Europe genotype; High-throughput sequencing

PMID:
29421305
DOI:
10.1016/j.virusres.2018.01.012
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center