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Carbohydr Polym. 2018 Apr 1;185:120-126. doi: 10.1016/j.carbpol.2017.12.077. Epub 2018 Jan 5.

Physicochemical characterization of polysaccharides from Chlorella pyrenoidosa and its anti-ageing effects in Drosophila melanogaster.

Author information

1
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002,China; School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
2
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002,China.
3
National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
4
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002,China; National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address: liubin618@hotmail.com.
5
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002,China; Department of Chemistry, University of California, Davis 95616, USA; Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou 362000, China. Electronic address: zhaochao@fafu.edu.cn.

Abstract

The physicochemical characteristics and in vitro antioxidant and in vivo anti-ageing activities of partial purified Chlorella pyrenoidosa polysaccharides (PCPPs) were investigated. The building blocks of PCPPs were mainly composed of D-glucose, D-galactose and D-mannose. The average molecular weight of PCPPs was 9,950 Da. In vitro antioxidant activity assays showed that PCPPs could effectively scavenge hydroxyl, 1,1-diphenyl-2-picrylhydrazyl, and superoxide radicals, with stronger effect on hydroxyl radicals. Furthermore, the mean lifespan of the male and female Drosophila melanogaster was extended by 11.5% and 10.6%, respectively. This was accompanied by an increase in the total activity of the endogenous antioxidant enzymes, superoxide dismutase, glutathione peroxidase, and catalase in young or old D. melanogaster administered with PCPPs. Moreover, a gender-dependent difference was observed both in lifespan and antioxidant enzyme activities in D. melanogaster. The results indicated that C. pyrenoidosa polysaccharides are potential natural antioxidants in extending lifespan.

KEYWORDS:

Anti-ageing; Chlorella pyrenoidosa polysaccharides; Drosophila melanogaster; Physicochemical characterization

PMID:
29421048
DOI:
10.1016/j.carbpol.2017.12.077
[Indexed for MEDLINE]

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