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Nano Lett. 2018 Jun 13;18(6):3322-3327. doi: 10.1021/acs.nanolett.8b00003. Epub 2018 Feb 12.

All Inkjet-Printed Amperometric Multiplexed Biosensors Based on Nanostructured Conductive Hydrogel Electrodes.

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School of Electronic Science and Engineering, Collaborative Innovation Center of Advanced Microstructures , Nanjing University , Nanjing 210093 , China.
Materials Science and Engineering Program and Department of Mechanical Engineering , The University of Texas at Austin , Austin , Texas 78712 , United States.


Multiplexing, one of the main trends in biosensors, aims to detect several analytes simultaneously by integrating miniature sensors on a chip. However, precisely depositing electrode materials and selective enzymes on distinct microelectrode arrays remains an obstacle to massively produced multiplexed sensors. Here, we report on a "drop-on-demand" inkjet printing process to fabricate multiplexed biosensors based on nanostructured conductive hydrogels in which the electrode material and several kinds of enzymes were printed on the electrode arrays one by one by employing a multinozzle inkjet system. The whole inkjet printing process can be finished within three rounds of printing and only one round of alignment. For a page of sensor arrays containing 96 working electrodes, the printing process took merely ∼5 min. The multiplexed assays can detect glucose, lactate, and triglycerides in real time with good selectivity and high sensitivity, and the results in phosphate buffer solutions and calibration serum samples are comparable. The inkjet printing process exhibited advantages of high efficiency and accuracy, which opens substantial possibilities for massive fabrication of integrated multiplexed biosensors for human health monitoring.


biosensor; hydrogel; inkjet printing; multiplex; polyaniline

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