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Front Cell Infect Microbiol. 2018 Jan 24;8:10. doi: 10.3389/fcimb.2018.00010. eCollection 2018.

Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection.

Author information

1
Department of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry, Osaka, Japan.
2
Division of Special Care Dentistry, Osaka University Dental Hospital, Osaka, Japan.
3
Department of Dermatology, Chiba University Graduate School of Medicine, Chiba, Japan.
4
Division of Microbiology and Infectious Diseases, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.

Abstract

Streptococcus pyogenes is responsible for a wide variety of cutaneous infections ranging from superficial impetigo to fulminant invasive necrotizing fasciitis. Dysfunction of desmosomes is associated with the pathogenesis of cutaneous diseases. We identified streptococcal pyrogenic exotoxin B (SpeB) as a proteolytic factor that cleaves the extracellular domains of desmoglein 1 and 3. In an epicutaneous infection model, lesional skin infected with an speB deletion mutant were significantly smaller as compared to those caused by the wild-type strain. Furthermore, immunohistological analysis indicated cleavage of desmogleins that developed around the invasion site of the wild-type strain. In contrast, the speB mutant was preferentially found on the epidermis surface layer. Taken together, our findings provide evidence that SpeB-mediated degradation of desmosomes has a pathogenic role in development of S. pyogenes cutaneous infection.

KEYWORDS:

SpeB; Streptococcus pyogenes; cutaneous infection; desmogleins; epidermal barrier

PMID:
29416987
PMCID:
PMC5787553
DOI:
10.3389/fcimb.2018.00010
[Indexed for MEDLINE]
Free PMC Article

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