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Leg Med (Tokyo). 2018 Mar;31:74-77. doi: 10.1016/j.legalmed.2018.01.005. Epub 2018 Feb 1.

A validation study of DNA methylation-based age prediction using semen in forensic casework samples.

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Forensic DNA Division, National Forensic Service, Wonju 26460, Republic of Korea.
Department of Forensic Medicine, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
Forensic DNA Division, National Forensic Service, Wonju 26460, Republic of Korea. Electronic address:


Previously, an age-predictive method based on DNA-methylation patterns in semen was developed, using three CpG sites (cg06304190 in the TTC7B gene, cg12837463, and cg06979108 in the NOX4 gene). Before considering the routine use of a new method in forensics, validation studies such as concordance and sensitivity tests are essential for obtaining expanded and more reliable forensic information. Here, we evaluated a previously described age-predictive method for semen for routine forensic use. Concordance testing showed a high correlation between the predicted and chronological age, with a mean absolute deviation from the chronological age of 4.8 years. Sensitivity testing suggested that age prediction with reliable accuracy and consistency was possible with >5 ng of bisulfite-converted DNA. We also confirmed the applicability of the age-predictive method in forensic casework, using forensic samples. Thus, the proposed method could serve as a very valuable forensics tool for accurate age prediction with semen samples.


Age prediction; DNA methylation; Forensic casework sample; Semen; Validation study

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