Format

Send to

Choose Destination
Leg Med (Tokyo). 2018 Mar;31:74-77. doi: 10.1016/j.legalmed.2018.01.005. Epub 2018 Feb 1.

A validation study of DNA methylation-based age prediction using semen in forensic casework samples.

Author information

1
Forensic DNA Division, National Forensic Service, Wonju 26460, Republic of Korea.
2
Department of Forensic Medicine, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
3
Forensic DNA Division, National Forensic Service, Wonju 26460, Republic of Korea. Electronic address: neobios@korea.kr.

Abstract

Previously, an age-predictive method based on DNA-methylation patterns in semen was developed, using three CpG sites (cg06304190 in the TTC7B gene, cg12837463, and cg06979108 in the NOX4 gene). Before considering the routine use of a new method in forensics, validation studies such as concordance and sensitivity tests are essential for obtaining expanded and more reliable forensic information. Here, we evaluated a previously described age-predictive method for semen for routine forensic use. Concordance testing showed a high correlation between the predicted and chronological age, with a mean absolute deviation from the chronological age of 4.8 years. Sensitivity testing suggested that age prediction with reliable accuracy and consistency was possible with >5 ng of bisulfite-converted DNA. We also confirmed the applicability of the age-predictive method in forensic casework, using forensic samples. Thus, the proposed method could serve as a very valuable forensics tool for accurate age prediction with semen samples.

KEYWORDS:

Age prediction; DNA methylation; Forensic casework sample; Semen; Validation study

PMID:
29413993
DOI:
10.1016/j.legalmed.2018.01.005
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center