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Curr Opin Cell Biol. 2017 Dec;49:108-115. doi: 10.1016/j.ceb.2018.01.002. Epub 2018 Feb 2.

Tracking the origin, development, and differentiation of hematopoietic stem cells.

Author information

1
Division of Hematology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA.
2
Division of Hematology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA 02138, USA.
3
Division of Hematology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Boston College, Chestnut Hill, MA 02467, USA.
4
Division of Hematology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA 02138, USA; Dana-Farber/Harvard Cancer Center, Boston, MA 02115, USA. Electronic address: dshah@research.bwh.harvard.edu.

Abstract

PURPOSE OF REVIEW:

The hierarchical nature of the hematopoietic system provides an ideal model system to illustrate the features of lineage tracing. We have outlined the utility of lineage tracing methods in establishing the origin and development of hematopoietic cells.

RECENT FINDINGS:

Methods such as CRISPR/Cas9, Polylox barcoding, and single-cell RNA-sequencing have improved our understanding of hematopoiesis.

SUMMARY:

This review chronicles the fate of the hematopoietic cells emerging from the mesoderm that subsequently develops into the adult blood lineages. Specifically, we explain classic techniques utilized in lineage tracing for the hematopoietic system, as well as novel state-of-the-art methods to elucidate clonal hematopoiesis and cell fate mapping at a single-cell level.

PMID:
29413969
PMCID:
PMC5821519
DOI:
10.1016/j.ceb.2018.01.002
[Indexed for MEDLINE]
Free PMC Article

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