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Nutr Metab (Lond). 2018 Jan 23;15:8. doi: 10.1186/s12986-018-0246-2. eCollection 2018.

Metabolomics insights into the modulatory effects of long-term compound polysaccharide intake in high-fat diet-induced obese rats.

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1School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006 People's Republic of China.
2School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510407 People's Republic of China.
Infinitus (China) Company Ltd, Guangzhou, 510623 China.



Polysaccharides can alleviate obesity in mammals; however, studies on mechanism of this alleviation are limited. A few studies have indicated that polysaccharides improve obesity by regulating the metabolism of the body. Therefore, a metabolomics approach, consisting of high resolution nuclear magnetic resonance (NMR) spectroscopy and a multivariate statistical technique, was applied to explore the mechanism of the protective effects of lentinan and Flos Lonicera polysaccharides (LF) on high-fat diet (HFD) induced obesity.


In this study, rats were randomly divided into three groups: control diet (CD), HFD, and HFD supplemented with a mixture of lentinan and Flos Lonicera polysaccharide. Histopathological and clinical biochemical assessments were also conducted. A combination of a NMR metabolomics study and a multivariable statistical analysis method to distinguish urinary and fecal metabolites was applied.


Significant obesity symptoms appeared in HFD rats (for example, significant weight gain, epididymal adipose accumulation and lipid deposition in hepatocytes), which was attenuated in the LF group. Additionally, the HFD induced a reduction of choline, citrate, pyruvate and glycerol and increased the levels of trimethylamine oxide (TMAO) and taurine. Of note, these metabolic disorders were reversed by LF intervention mainly through pathways of energy metabolism, choline metabolism and gut microbiota metabolism.


LF supplementation had a re-balancing effect on the disturbed metabolic pathways in the obese body. The results of this study validate the therapeutic effect of the compound polysaccharide--LF in obesity and described the biochemical and metabolic mechanisms involved.


Flos Lonicera polysaccharides(FLP); Lentinan; Metabolomics; NMR; Obesity

Conflict of interest statement

All of the animal experiments in this study were approved by the Institutional Animal Care and Use Committee of Sun Yat-sen University.Not applicable.The authors declare no competing financial interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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