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Emerg Microbes Infect. 2018 Feb 7;7(1):14. doi: 10.1038/s41426-017-0012-y.

Two reassortant types of highly pathogenic H5N8 avian influenza virus from wild birds in Central China in 2016.

Ma L1,2,3, Jin T4, Wang H1, Liu H1, Wang R1, Li Y5, Yang G5, Xiong Y5, Chen J5, Zhang J5, Chen G5, Li W6, Liu D1,2,3,6, Lin P4, Huang Y4, Gao GF2,3,6,7, Chen Q8,9.

Author information

1
CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, Hubei, China.
2
Center for Influenza Research and Early-Warning (CASCIRE), Chinese Academy of Sciences, Beijing, 100101, China.
3
University of Chinese Academy of Sciences, Beijing, 100049, China.
4
China National Genebank-Shenzhen, BGI-Shenzhen, Shenzhen, 518120, China.
5
The monitoring center of wildlife diseases and resource of Hubei, Wuhan, 430071, China.
6
Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
7
Office of Director-General, ChineseCenter for Disease Control and Prevention (China CDC), Beijing, 102206, China.
8
CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, Hubei, China. chenqj@wh.iov.cn.
9
Center for Influenza Research and Early-Warning (CASCIRE), Chinese Academy of Sciences, Beijing, 100101, China. chenqj@wh.iov.cn.

Abstract

Since 2016, the highly pathogenic avian influenza H5N8 virus has emerged in the Central Asian flyway and Europe, causing massive deaths in poultry and wild birds. In this study, we isolated and identified three H5N8 viruses from swan goose and black swans in Hubei province during the 2016/2017 winter season. Whole-genome sequencing and phylogenetic analysis revealed that the three viruses clustered into a group of H5N8 viruses from Qinghai Lake and Europe. A novel reassortment virus from swan goose was distinguished from that of black swans, in that its PA and NP genes were distinct from those of Qinghai Lake viruses. Molecular dating revealed that the ancestral strain of these H5N8 viruses emerged around July 2015. From sequence comparison, we discovered eight amino acid substitutions in HA and NA during the adaption process from poultry to wild birds. The three viruses were isolated from wild birds in the East Asian-Australasian flyway; however, the viral genomes were similar to H5N8 viruses circulating along the Central Asian flyway. From these data, we conclude that wetlands and lakes in Central China may play a key role in disseminating H5N8 viruses between the East Asian-Australasian and Central Asian flyways.

PMID:
29410395
PMCID:
PMC5837153
DOI:
10.1038/s41426-017-0012-y
[Indexed for MEDLINE]
Free PMC Article

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