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J Struct Biol. 2018 Jun;202(3):200-209. doi: 10.1016/j.jsb.2018.02.001. Epub 2018 Feb 3.

Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion.

Author information

1
Spanish National Research Council (CNB-CSIC), Darwin 3, 28049 Madrid, Spain. Electronic address: jj.fernandez@csic.es.
2
Dept. Biochemistry and Biophysics, University of California, San Francisco, USA.
3
MRC Laboratory of Molecular Biology, Francis Crick Avenue Cambridge CB2 0QH, UK; Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK.

Abstract

Recent evidence suggests that the beam-induced motion of the sample during tilt-series acquisition is a major resolution-limiting factor in electron cryo-tomography (cryoET). It causes suboptimal tilt-series alignment and thus deterioration of the reconstruction quality. Here we present a novel approach to tilt-series alignment and tomographic reconstruction that considers the beam-induced sample motion through the tilt-series. It extends the standard fiducial-based alignment approach in cryoET by introducing quadratic polynomials to model the sample motion. The model can be used during reconstruction to yield a motion-compensated tomogram. We evaluated our method on various datasets with different sample sizes. The results demonstrate that our method could be a useful tool to improve the quality of tomograms and the resolution in cryoET.

KEYWORDS:

Beam-induced sample motion; Electron cryo-tomography; Subtomogram averaging; Tilt-series alignment; Tomographic reconstruction

PMID:
29410148
PMCID:
PMC5949096
DOI:
10.1016/j.jsb.2018.02.001
[Indexed for MEDLINE]
Free PMC Article

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