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Exp Gerontol. 2018 Apr;104:127-137. doi: 10.1016/j.exger.2018.01.016. Epub 2018 Feb 2.

Differential response to caloric restriction of retroperitoneal, epididymal, and subcutaneous adipose tissue depots in rats.

Author information

1
Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Japan; Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Noda, Japan. Electronic address: 3B15706@ed.tus.ac.jp.
2
Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Japan; Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Noda, Japan. Electronic address: kobayashim@rs.tus.ac.jp.
3
Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Japan. Electronic address: 3B14610@ed.tus.ac.jp.
4
Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Japan. Electronic address: 3B17605@ed.tus.ac.jp.
5
Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Japan. Electronic address: j3a12030@ed.tus.ac.jp.
6
Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Noda, Japan. Electronic address: ysudo@rs.tus.ac.jp.
7
Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Noda, Japan; Department of Internal Medicine Research, Sasaki Institute, Sasaki Foundation, Tokyo, Japan. Electronic address: nmsokita@gmail.com.
8
Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Japan; Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Noda, Japan. Electronic address: higami@rs.noda.tus.ac.jp.

Abstract

The beneficial actions of caloric restriction (CR) are partially mediated by metabolic remodeling of white adipose tissue (WAT). Recently, we showed that CR enhances de novo fatty acid (FA) biosynthesis and mitochondrial biogenesis, particularly in WAT. Here, to better understand the response of WAT to CR, we compare the effects of CR on three WAT depots in rats: retroperitoneal (rWAT), epididymal (eWAT) and subcutaneous (sWAT). Computed tomography and histological analysis showed that CR reduced the volume and average size of rWAT adipocytes. In all WAT depots, CR markedly upregulated the expression of proteins involved in FA biosynthesis in fed rats. In visceral WAT (rWAT and eWAT), hormone-sensitive lipase (lipolytic form) phosphorylation was increased by CR under fed conditions, and decreased by CR under fasted conditions. Conversely, in sWAT, hormone-sensitive lipase phosphorylation was increased by CR under fasted conditions. CR enhanced the effect of feeding on AKT activity in sWAT (indicative of a positive effect on insulin sensitivity) but not in rWAT or eWAT. These data suggest that CR improves lipid metabolism in an insulin signaling-dependent manner in sWAT only. The effects of CR on adipokine (adiponectin and leptin) expression were also different among rWAT, eWAT and sWAT, and CR reduced the gene expression of M2 macrophage markers in rWAT and sWAT, but not in eWAT. We conclude that CR differentially affects the characteristics of WAT depots in rats, including adipocyte size, lipid metabolism, insulin signaling, adipocytokine profile and macrophage infiltration.

KEYWORDS:

Caloric restriction; Insulin signaling; Lipid metabolism; Macrophage; White adipose tissue

PMID:
29410017
DOI:
10.1016/j.exger.2018.01.016
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