Format

Send to

Choose Destination
Mol Cell Endocrinol. 2018 Sep 15;473:217-224. doi: 10.1016/j.mce.2018.01.022. Epub 2018 Feb 2.

Prostaglandin E2 (PGE2) is a testicular peritubular cell-derived factor involved in human testicular homeostasis.

Author information

1
Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Ludwig-Maximilian-University (LMU), D-82152 Planegg, Germany. Electronic address: v.rey@lrz.uni-muenchen.de.
2
Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Ludwig-Maximilian-University (LMU), D-82152 Planegg, Germany; Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina. Electronic address: rossi@dna.uba.ar.
3
Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Ludwig-Maximilian-University (LMU), D-82152 Planegg, Germany. Electronic address: kim.dietrich@lrz.uni-muenchen.de.
4
Andrologicum, D-80331 Munich, Germany. Electronic address: info@andrologicum.com.
5
Andrology-Center, D-81241 Munich, Germany. Electronic address: j.u.schwarzer@gmx.de.
6
Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Ludwig-Maximilian-University (LMU), D-82152 Planegg, Germany. Electronic address: welter@lrz.uni-muenchen.de.
7
Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina. Electronic address: mfrungieri@dna.uba.ar.
8
Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Ludwig-Maximilian-University (LMU), D-82152 Planegg, Germany. Electronic address: Mayerhofer@lrz.uni-muenchen.de.

Abstract

In man, blockage of prostaglandin (PG)-production e.g. by non-steroidal anti-inflammatory drug (NSAIDs) may have negative testicular side effects, implying beneficial actions of PGs in the testis. We examined human testicular samples and isolated human testicular peritubular cells (HTPCs) to explore sites of PG-synthesis and targets. HTPCs express cyclooxygenase 1 (COX1) and secrete PGE2. Receptors (EP1, 2, 4) were specifically identified in peritubular cells. In HTPCs PGE2 significantly increased mRNA levels of the contractility protein calponin, but did not induce contractions. PGE2, as well as EP1 and EP4 receptor agonists, significantly increased glia cell line derived neurotrophic factor (GDNF) mRNA and/or protein levels. Importantly, the NSAID ibuprofen reduced PGE2 and this action also lowered SMA and calponin mRNA levels and levels of secreted GDNF protein. The results reveal an unknown PGE2 system in the human testis, in involving peritubular cells, which may be prone to interference by NSAIDs.

KEYWORDS:

Anti-inflammatory drugs; GDNF; Male fertility; Male infertility; Testis

PMID:
29408603
DOI:
10.1016/j.mce.2018.01.022

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center