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Colloids Surf B Biointerfaces. 2018 Mar 1;163:412-418. doi: 10.1016/j.colsurfb.2018.01.002. Epub 2018 Jan 3.

Electrodeposition to construct mechanically robust chitosan-based multi-channel conduits.

Author information

1
Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.
2
Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Lab of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
3
Department of Hematology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
4
CNRS UMR 7561 and FR CNRS-INSERM 32.09 Nancy University, Vandœuvre-lès-Nancy, France.
5
Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, Hubei Key Lab of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China. Electronic address: shixwwhu@163.com.
6
Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China; Hubei Province Key Laboratory of Allergy and Immune Related Diseases, Wuhan University, Wuhan 430071, China. Electronic address: yunchen@whu.edu.cn.

Abstract

A series of electrodeposited chitosan-based multi-channel conduits (ECMC) with potential for peripheral nerve tissue engineering were constructed using a novel electrodeposition method combined with homemade molds. The structural and mechanical properties of the ECMC were characterized by scanning electron microscopy, Fourier-transformed infrared spectroscopy, X-ray diffraction patterns and mechanical testing. The results showed that the electrodeposition process did not change the chemical structure of the chitosan molecules, but endowed the ECMC with high levels of flexibility and elasticity. Hemocompatibility and cytocompatibility of the ECMC were evaluated by hemolysis assay, MTT assay and live/dead assay. The results indicated that the ECMC had a low hemolysis rate, and can promote cell proliferation and support cell adhesion. This work provides a safe and feasible electrodeposition method to construct chitosan-based conduits with potential applications for peripheral nerve tissue engineering.

KEYWORDS:

Chitosan; Electrodeposition; Multi-channel conduits; Peripheral nerve tissue engineering

PMID:
29408165
DOI:
10.1016/j.colsurfb.2018.01.002
[Indexed for MEDLINE]

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