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J Pharm Anal. 2014 Apr;4(2):125-131. doi: 10.1016/j.jpha.2013.08.001. Epub 2013 Aug 14.

Reagent-free determination of amikacin content in amikacin sulfate injections by FTIR derivative spectroscopy in a continuous flow system.

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Departamento de Análisis y Control, Facultad de Farmacia y Bioanálisis, Universidad de Los Andes, Mérida 5101A, Venezuela.
Laboratorio de Espectroscopia Molecular, Departamento de Química, Facultad de Ciencias, Universidad de Los Andes, Mérida 5101A, Venezuela.


The quantitative estimation of amikacin (AMK) in AMK sulfate injection samples is reported using FTIR-derivative spectrometric method in a continuous flow system. Fourier transform of mid-IR spectra were recorded without any sample pretreatment. A good linear calibration (r>0.999, %RSD<2.0) in the range of 7.7-77.0 mg/mL was found. The results showed a good correlation with the manufacturer's and overall they all fell within acceptable limits of most pharmacopoeial monographs on AMK sulfate.


Amikacin; Continuous flow system; FTIR derivative spectrometry; Injection; Pharmaceutical preparation; Sulfate

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