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Molecules. 2018 Feb 5;23(2). pii: E332. doi: 10.3390/molecules23020332.

Anti-Inflammatory Effect of Melittin on Porphyromonas Gingivalis LPS-Stimulated Human Keratinocytes.

Author information

1
Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, 42472, Republic of Korea. kimwoonhae@cu.ac.kr.
2
Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, 42472, Republic of Korea. ahj119@cu.ac.kr.
3
Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, 42472, Republic of Korea. jy1118@cu.ac.kr.
4
Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, 42472, Republic of Korea. daldy99@naver.com.
5
Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, 42472, Republic of Korea. asdf8760@naver.com.
6
Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, 42472, Republic of Korea. minzy1081@cu.ac.kr.
7
Department of Pathology, College of Medicine, Dongguk University, Gyeongju-si, 38066, Republic of Korea. minkyungk76@naver.com.
8
Department of Agricultural Biology, National Academy of Agricultural Science, RDA, 300, Nongsaengmyeong-ro, Wansan-gu, Jeonju-si, Jeollabuk-do 55365, Republic of Korea. sangmih@korea.kr.
9
Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, 42472, Republic of Korea. kkpark@cu.ac.kr.

Abstract

Periodontitis is a chronic inflammatory disease that contributes to the destruction of the gingiva. Porphyromonas gingivalis (P. gingivalis) can cause periodontitis via its pathogenic lipopolysaccharides (LPS). Melittin, a major component of bee venom, is known to have anti-inflammatory and antibacterial effects. However, the role of melittin in the inflammatory response has not been elucidated in periodontitis-like human keratinocytes. Therefore, we investigated the anti-inflammatory effects of melittin on a P. gingivalis LPS (PgLPS)-treated HaCaT human keratinocyte cell line. The cytotoxicity of melittin was measured using a human keratinocyte cell line, HaCaT, and a Cell Counting Kit-8. The effect of melittin on PgLPS-induced inflammation was determined with Western blot, real-time quantitative PCT, and immunofluorescence. PgLPS increased the expression of toll-like receptor (TLR) 4 and proinflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-8, and interferon-γ (IFN-γ). Moreover, PgLPS induced activation of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), extracellular signal-regulated kinase (ERK), and protein kinase B/Akt. Melittin also inhibited the expression of proinflammatory cytokines by suppressing the activation of the NF-κB signaling pathway, ERK, and Akt. Melittin attenuates the PgLPS-induced inflammatory response and could therefore be applied in the treatment of periodontitis for anti-inflammatory effects.

KEYWORDS:

Keratinocytes; Porphyromonas gingivalis; lipopolysaccharides; melittin; periodontitis

PMID:
29401750
PMCID:
PMC6017529
DOI:
10.3390/molecules23020332
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

The authors declare no conflict of interest.

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