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Metab Syndr Relat Disord. 2018 Mar;16(2):90-96. doi: 10.1089/met.2017.0138. Epub 2018 Feb 2.

Relationship Between Paraoxonase-1 and Butyrylcholinesterase Activities and Nutritional Status in Mexican Children.

Author information

1
1 Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional (CIIDIR) Unidad Michoacán, Instituto Politécnico Nacional, Michoacán, Mexico .
2
2 Facultad de Ciencias del Deporte, Universidad Autónoma del Estado de Morelos , Cuernavaca, México .
3
3 División Ciencias Agropecuarias e Ingenierías de la Universidad Popular de la Chontalpa , Cárdenas, Tabasco, Mexico .
4
4 Laboratorio de Género, Salud y Ambiente, Facultad de Medicina, Universidad Autónoma de San Luis Potosí , San Luis Potosí, México .
5
5 Laboratorio de Neuroprotección, Facultad de Farmacia, Universidad Autónoma del Estado de Morelos , Cuernavaca, Mexico .

Abstract

BACKGROUND:

The enzymes butyrylcholinesterase (BuChE) and paraoxonase-1 (PON1) are the primary bioscavenging enzymes in serum and exhibit antioxidant and anti-inflammatory activities. PON1 has been associated with diseases caused by high oxidative stress, whereas BuChE appears to be involved in the pathophysiology of the metabolic syndrome and related disorders. It has been suggested that children from rural communities in Mexico may have a predisposition to develop obesity or type 2 diabetes during adolescence or adulthood. The objective of this study was to determine whether associations exist between the paraoxonase (PONase)/arylesterase (AREase) activity of PON1, its PON1-Q192R and PON1-L55M polymorphisms, and BuChE activity with the nutritional status and lipid profiles in a group of children from rural communities in Mexico.

METHODS:

A group of 97 boys and girls from a rural community in Mexico were assessed for body mass index, the enzymatic activities of BuChE, PONase, and AREase were measured in serum, and their lipid profiles were determined. Genetic polymorphisms of PON1-L55M and PON1-Q192R were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

RESULTS:

The children were classified into four groups: thinness, normal weight, overweight, and obese. Of the children studied, 34.4% were overweight and obese. The mean age of the participants was 9.5 years (standard deviation = 1.8). The L allele of the PON1-L55M genotype was the most frequent (83.3%), and the R allele of the PON1-Q192R genotype was the most frequent (61.8%). Overweight and obese children had higher values of BuChE, total cholesterol, triglycerides (TG), and lower high-density lipoprotein (HDL-C) values than children with thinness or normal weight (P = 0.028, P = 0.019, P = 0.004, P = 0.069 and P = 0.021, respectively). The levels of AREase and PONase and the prevalence of PON1-L55M and PON1-Q192R genotypes were similar between groups (P = 0.484 and P = 0.380, respectively).

CONCLUSIONS:

This study establishes a positive association of BuChE activity with nutritional status and serum TG.

KEYWORDS:

PON1 polymorphism; butyrylcholinesterase; obesity; overweight; paraoxonase

PMID:
29393817
DOI:
10.1089/met.2017.0138
[Indexed for MEDLINE]

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